14
5061-049 REV 1.4
25-9574-75UM AE 05/2010
14
4. LIFE SCIENCE
4.1. File system
The Life Science Screen is organized into six sub-folders as shown below:
Folder Keypad Number
Application
Function
1
DNA
Concentration and purity check for DNA samples
2
RNA
Concentration and purity check for RNA samples
3
Oligo
Concentration and purity check for Oligonucleotide samples
4
Tm Calculation
DNA melting point calculator
5
Cy Dye
Labeling efficiency measurement
6
Protein
Folder containing methods for protein determination
Pressing 6 enters the Protein sub-folder which contains various standard methods
for protein determination:-
4.2. Nucleic Acids
4.2.1. Theory
• Nucleic acids can be quantified at 260 nm because at this wavelength there
is a clearly defined peak maximum. A 50 µg/ml DNA solution, a 40 µg/ml RNA
solution and 33 µg/ml solution of a typical synthetic Oligonucleotide all have an
optical density of 1.0 A in a 10 mm pathlength cell. These factors (50, 40 and 33
respectively) can be inserted into the formula (1) below, although they do vary
with base composition and this can be calculated more precisely if the base
sequence is known.
(1) Concentration = Abs260 * Factor (where * means “multiplied by”)
