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Life Science
Group

    Sig 1212

10002468 Rev D     US/EG

Bio-Rad 
Laboratories, Inc.

Web site 

www.bio-rad.com  

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800 424 6723  

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Summary of Contents for PowerPac HV Series

Page 1: ...PowerPac HV Power Supply Instruction Manual Catalog Numbers 164 5056 and 164 5059...

Page 2: ......

Page 3: ...Pac HV displays the software version momentarily after switching the power ON State clearly the error code error message or anomaly and the conditions that originated the problem including run paramet...

Page 4: ......

Page 5: ...er supply must be kept away from wet surfaces Avoid splashing or leaking solutions into the power supply 3 Always connect the power supply to a grounded AC outlet An acceptable power cord is provided...

Page 6: ......

Page 7: ...un in Manual Mode 12 4 2 Basic Methods Mode 14 4 2 1 Create Edit and Run a Basic Method 15 4 3 IEF Methods Mode 18 4 3 1 Create Edit and Run an IEF Method 19 4 4 Temperature Mode 22 4 4 1 Create Edit...

Page 8: ...ds Connected Incorrectly 5 Figure 7 Menu Overview for Manual Operation 11 Figure 8 Menu Overview for Basic Methods Mode 14 Figure 9 Menu Overview for IEF Methods 18 Figure 10 Menu Overview for Tempera...

Page 9: ...imiting parameter Output range Voltage 20 5 000 Volts V Adjustable in 0 1 V increments from 20 0 to 99 9 V Adjustable in 1 V increments from 100 to 5000 V Current 0 05 500 mA Adjustable in 0 01 mA ste...

Page 10: ...editing running parameters Automatic detection of no load short circuit rapid resistance change ground leak fan failure and system overheating Automatic completion if desired of a run interrupted by...

Page 11: ...with the carrier in accordance with their instructions before contacting Bio Rad Laboratories Unpack the PowerPac HV Remove the plastic film from the translucent green top case The plastic film may le...

Page 12: ...time mode hours volt hours or untimed Up down Used to scroll through method list or method protocol An asterisk is arrows and used to identify the selected method or step The up arrow is used to deci...

Page 13: ...r cord rear of the instrument Connect the other end to a compatible power source 2 Connect cells Insert power leads into one of the output terminals located on the front of the power supply shown to t...

Page 14: ...enable and disable safety features and infrared IR port as well as LCD display contrast adjustment key sound and set local time and date Step Procedure Description 1 Open the Setup editor Press SETUP...

Page 15: ...corner of the Run screen Warning Turning the power supply off Stopping a run in progress with PFd enabled is regarded as a power failure The interrupted run will resume automatically when the power i...

Page 16: ...ound and the error code 01 to be displayed see Section 6 3 For example if an electrophoresis cell is not connected or the current is too low less than 0 02mA for Manual or Basic Methods and 0 005mA fo...

Page 17: ...when Temperature Probe Check Default the temperature probe is connected OFF ON The run is paused and error code 18 is displayed in case of failure A failure occurs when the temperature remains 5 C les...

Page 18: ...IEF Methods Nine 9 methods with up to nine 9 steps can be stored This mode allows direct programming for high voltage low current applications also allows voltage ramping and run delay see Section 4...

Page 19: ...rform a run in Manual mode Fig 7 Menu Overview for Manual Mode EDIT LIMITS 100 V 500 mA 400W TIME UNTIMED _______ CONSTV LIMITA LIMITW S1 1 EDIT TIME 200 V 500 mA 500W TIME UNTIMED____________ HRS VHR...

Page 20: ...the Use the soft keys to select a nonconstant nonconstant parameters parameter and the keypad to enter its value Press EDIT key twice to go back to step 3 CONSTV A or W Press CONST soft key to change...

Page 21: ...the elapsed time versus the programmed time timed runs elapsed time untimed run voltage current and power values 8 Pause Press the soft key PAUSE or the key to pause the run in progress The run param...

Page 22: ...__________ OPEN DELETE NEW METHOD SDS PAGE S1 10V 00 30 HRS S2 200V UNTIMED S3 EMPTY ________________________ METHODS DELETE SAVE S1 1 EDIT LIMITS 200 V 500 mA 400W TIME UNTIMED _______ CONSTV LIMITA...

Page 23: ...ed method Select a stored method using the arrow scroll keys and press the DELETE Soft key An asterisk indicates the selected method Note If the method list is full nine methods a method needs to be d...

Page 24: ...Operates with constant current CONSTW Operates with constant power Press EDIT to continue 7 Enter limits for the Use the soft keys to select a parameter and the non constant alphanumerical keypad to...

Page 25: ...the run Press to start a run 11 Monitor the run The RUN screen is used to monitor run parameters or to pause a run The current step Volts mA and Watts values elapsed time versus the programmed time an...

Page 26: ...ig 9 Menu Overview for IEF Methods IEF METHOD LIST pH 3 10 IEF EMPTY ____________________________________ OPEN DELETE NEW METHOD PH 3 10 S1 100V 00 30 HRS S2 800V 05 30 HRS S3 EMPTY ________ METHOD DE...

Page 27: ...ow scroll keys an asterisk indicates the selected method and press the OPEN soft key Go to step 4 to edit method otherwise go to step 11 to start the run DELETE a stored method An asterisk indicates t...

Page 28: ...ected step 6 Set the Voltage profile Use the V MAX soft key to enter the maximum voltage value with the keypad for the current step Use the V SLOPE soft key to select the voltage ramping slow linear o...

Page 29: ...Add a new step or save To program additional steps press the down arrow the method key to select the empty step and repeat steps 5 9 If the method will not be saved start the run Press SAVE and then...

Page 30: ...ature to 90 C The PowerPac HV monitors the temperature of the electrophoresis cell through the temperature probe catalog 165 5058 attached to the wall of the cell The temperature of the cell is contro...

Page 31: ...on 3 3 4 for additional information on TEMP PROBE options 5 Set temperature limit Use the alphanumeric keypad to enter the desired temperature 30 90 C Press the EDIT soft key to continue 6 Enter param...

Page 32: ...y To edit the run parameters during PAUSE see step 6 To Edit preference see Section 3 Edits made during the run may be saved if SAVE soft key is pressed at the end of the run Otherwise the method will...

Page 33: ...nal run If the current step started as untimed it cannot be changed to hours or volt hours Fig 11 Menu Overview for Editing a Paused Method RUN S2 4 216 V 235 mA 50 W 00 02 00 30 32HRS____ PAUSE RUN P...

Page 34: ...or Manual run editing Section 4 2 for Basic Methods editing Section 4 3 for IEF Methods editing Section 4 4 for Temperature mode run editing Use the CONTINUE soft key or to resume the run 3 Exit the R...

Page 35: ...onal computer PC either directly from the power supply or through a PDA Using the PDA with PowerPac Remote methods can be created stored and beamed to and from the PowerPac HV The PDA may also be used...

Page 36: ...tem 400 MHz processor 256 MB RAM minimum 512 MB recommended 1 024 x 768 screen resolution with true color mode 24 or 32 bits 10 GB hard drive CD ROM drive Infrared IR port or adaptor Minimum PDA speci...

Page 37: ...he fuse may need to be replaced 1 Disconnect the power cord from the electrical outlet 2 Use a slot head screwdriver to press the tabs on the side of the fuse holder toward each other This will releas...

Page 38: ...e 2 Replace the fuse See Section 6 1 1 for details Repeatedly blown fuses Hardware failure Contact Bio Rad Technical Support Leads from the cell are Some leads are not The PowerPac HV not not long eno...

Page 39: ...levels are wrong connections appropriate ERROR STOP CODE 03 Connection to an Press the CONTINUE soft OVER VOLTAGE external source or key to continue the run CORRECT AND CONTINUE control circuitry RUN...

Page 40: ...chiller system for leaks Verify that the electrophoresis cell rests on an insulated and dry surface Check power source connections Additional capacitance from external EM filters or an uninterruptibl...

Page 41: ...Hardware Turn power supply off and on then 19 99 malfunction restart run If problem persists contact Bio Rad Technical Support Contacting Technical Support For Technical Support Contact your local Bi...

Page 42: ...Real time clock Yes Automatic recovery after power failure Yes user selectable setup values maintained Data transfer archiving Yes Temperature control Yes via temperature probe 30 90 C 2 C Microampere...

Page 43: ...by Bio Rad Laboratories for this power supply Deliberate or accidental misuse Damage caused by disaster For inquiry or request for repair service contact your local Bio Rad office Warranty Informatio...

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Page 48: ...rmany 089 31 884 0 Greece 30 210 9532 220 Hong Kong 852 2789 3300 Hungary 36 1 459 6100 India 91124 4029300 Israel 03 963 6050 Italy 39 02 216091 Japan 03 6361 7000 Korea 82 2 3473 4460 Mexico 52 555...

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