Step Procedure
Description
13.
The Run Completed or Run Terminated screen is
displayed at the end of a run. Press
RERUN
to
repeat the run or
EXIT
to display the method step
list.
4.4 Temperature Mode
The Temperature operation mode is used to run an electrophoresis apparatus at constant
temperature. Temperature control is very useful to perform DNA sequencing and Single
Stranded Conformational Polymorphism (SSCP) analysis in the Sequi-Gen
®
GT system. The
temperature limit may be set from 30 to 90°C; actual temperature will be controlled from room
temperature to 90°C.
The PowerPac HV monitors the temperature of the electrophoresis cell through the
temperature probe (catalog #165-5058) attached to the wall of the cell. The temperature of
the cell is controlled by regulating the power output (in W) once the cell temperature is
within 2°C of the set temperature.
Fig. 10. Menu Overview for Temperature Mode
TEMPERATURE MODE
TO EXIT TEMP MODE
REMOVE THE PROBE
CONTINUE
SET TEMPERATURE LIMIT
50 °C
______________________
EDIT
EDIT TIME
55 W
5000V
500 mA
TIME: UNTIMED 50°C _
HRS
VHRS UNTIMED
EDIT PARAMETERS
55 W
5000 V
500 mA
TIME: UNTIMED 50°C _
LIMITV LIMITA CONSTW
RUN
216 V
235 mA
50 W
00:02/00:30 01:32HRS_____
PAUSE
EDIT
EDIT
Connect Temperature
Probe to rear of
instrument
22
PowerPac HV – Instruction Manual
