Section IV: Sequence Analysis FAQ Sheet
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CEQ™ 8000 Series Genetic Analysis System
Section IV: Sequence Analysis FAQ Sheet
General Operation
Can I perform DNA sequencing and fragment analysis
on the same plate using the CEQ 8000 Series System?
If you have purchased or have upgraded to the CEQ 8000 Series System it is possible
and practical to run both sequencing and fragment analysis on the same plate. The
CEQ 8000 Series System upgrade uses the same 33cm capillaries and plenum for both
sequencing and fragment analysis. It is still advisable to separate the sample plate into
blocks of sequencing and fragment samples if overall run time is critical. Separating
the different types of samples into blocks will reduce delays due to possible
temperature ramping between methods.
Can I switch to another database to inspect old data
while new data is being collected?
No, you may only retrieve data from the active database, and you may not switch the
active database while any of the other CEQ software modules (Run, Sample Setup, or
Analysis) are open. However, data from different projects within the same database
can be examined freely, even while new data is being collected.
What are the differences between the LFR-1, LFR-a, LFR-b and
LFR-c methods?.
You can examine all the parameters of each separation method by clicking the Method
tab in the CEQ Sample Setup module. The different methods are designed for different
read lengths and time constraints. LFR-1 is the standard Long fast read sequencing
method for the CEQ System. LFR-a, b and c are alternative methods that can be used,
depending on individual application and the desired amount of sequence data.
LFR-a typically yields the longest read lengths but also requires additional separation
time. LFR-b can yield approximately 700 bases in a shortened separation time
compared to the standard LFR-1. The LFR-b method greatly reduces the separation
time compared to LFR-a but at the cost of 50 or so bases. LFR-c is the fastest method,
reducing the separation time for applications that require only several hundred bases of
sequence data.
The choice of which method to use is dictated by the amount of sequence required and
the amount of time the researcher is willing to use. In addition, the researcher has the
option to create their own separation methods to better suit their individual needs.
