Section I: General Diagnostic Guidelines
Sequence Analysis Troubleshooting Guide
3
The software allows for the development of custom separation methods. If a
significantly higher separation voltage (6–12KV) is desired, then the advanced
features of assigning the separation conditions should be employed. The advanced
features allow for a gradual, stepped ramp to the final separation voltage. Such a
gradual voltage increase allows the linear polyacrylamide (LPA) to slowly heat up and
thereby reduces possible thermal expansion out of the capillary caused by the rapid
exposure to high voltages. An example of such a three step ramp is given in Figure 3.
Figure 3:
Example of a three step ramp
Current ramps up to the
final separation level in
three stages.
Current remains steady
throughout the rest of the
separation time
