Azure Cielo Real-Time PCR Systems User Manual
Page 7
1.2—Plates and Tube Compatibility
Azure Cielo Real-Time PCR system is compatible with:
A. Low-profile 96-well Plates
• Low-profile plates have wells of approximate height 15.5 mm (depending on manufacturer).
These modern designs reduce condensate formation and allow for accurate light detection in
fluorescence assays, low-volume reactions, and fast PCR.
• Non-skirted Plate: This plate lacks a surrounding panel. This format fits blocks of most thermal
cyclers and qPCR systems, but may not be suitable for robotic applications without stabilization.
• Semi-skirted Plate: This plate has a short panel around the edge of the plate, providing adequate
support during pipetting and mechanical strength for robotic handling.
*Azure Cielo Real-Time PCR Systems cannot accommodate a fully skirted 96-well plate.
Azure Cielo Systems are compatible with only a non-skirted or semi-skirted 96-well plate*
• Plate color can be White, Frosted or Clear. For assays requiring higher fluorescent sensitivity and
accuracy, white or frosted plates would be recommended.
• Plates should be sealed with Optical Grade Seal Film. Detection of this system is from above and
through the top of each well in the plate. Significant signal loss should be expected if not using
optically clear caps or seals.
• Plate rim (lip): The “rim” of a 96-well plate is a peripheral upper elevation around the plate.
Rimmed plates are
NOT
compatible with the Cielo system.
• Plate Notch: The notch is a modified corner of a 96-well plate. The Azure Cielo Systems are
versatile and can accommodate notching at any corner position. (A1,H1, A12 and H12)
*The Azure Cielo Systems are not compatible with any 384-well plate*