Azure Cielo Real-Time PCR Systems User Manual
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B. Setting and using the Gradient option:
• A gradient can be an extremely useful option, allowing the user to test several
different temperature conditions within a single run. The Cielo Real-Time PCR
system uses a gradient option that offers different temperatures by columns across
the 96-well plate. The gradient low temperature and high temperature limits are
determined by the user. The instrument software considers those limits, and then
empirically determines the temperature for each column across the plate. Users are
not able to alter those assigned temperatures and a minimum difference of 1 degree
is required for the temperature range
• The GRADIENT option can be accessed by opening the settings
within the step to
which the gradient needs to be added. The camera/gradient dialog box will appear.
To enable the gradient option, tap the toggle switch to turn the gradient function ON.
Enter the high and low temperatures that will define the range needed. Please note,
a minimum range of 1 degree is required. When the GRADIENT has been turned ON,
the switch button will turn BLUE. Select MODIFY
to save changes. To abort
the changes, select the CANCEL
option, which will return the user to the RUN
SETUP screen without saving changes.