122
Apr-09
Avantes
www.avantes.com
6.2.4
Direct Attach Cuvette Holders
The CUV- direct attachment cuvette holders are available for all our fiber optic light sources, for the
different models see technical data table. The direct attach cuvette holders can be used for
absorbance and fluorescence measurements.
They come with two 90 degree and a 180 degree 3/8"-24 thread that allows the COL-UV/VIS
collimating lens to be mounted for absorbance or fluorescence setups.
Two SiO
2
coated aluminum mirrors with 3/8"-24 thread screw are delivered with the direct attach
cuvette holders to mount in the other thread, to make the fluorescence signal even stronger.
The
cuvette holders also have a 5 mm wide filter slot.
For most of the AvaLight light sources special direct attach cuvette holders are available (see table)
that can be mounted directly on the front panel of the light source instead of the standard filter
holder.
Technical Data
CUV-DHc/XE/LED
CUV-DHS
CUV-HAL
Lightsource
AvaLight-
DHc
AvaLight-
XE
AvaLight
-LED
AvaLight-DH-S
AvaLight-HAL
Wavelength range
200-2500nm
Cuvette Dimensions
10 x 10 mm
Light source connection
SMA thread incl col. lens
Mounting plate
Mounting plate
Fiber connection
1 x COL-UV/VIS, SMA 905 connectors
Fluorescence mirrors
2 x SiO
2
coated aluminum mirrors
Filter slit
Max 5 mm wide
Dimensions
60 x 43 x 28 mm
60x 50 x
50mm
60x 35 x
35mm
The Direct Attach Cuvette Holders can be used for 2 types of applications, absorbance and
fluorescence
For
absorbance measurements
follow these instructions:
1.
Attach the cuvette holder directly to the SMA port of the light source
2.
Attach an SMA terminated optical fiber between the cuvette holder 180-degree port and the
spectrometer.
3.
Optionally install a filter by loosening the filter screw and insert the filter into the filter slot,
max filter size is 5 mm.
4.
The cuvette holders are designed to hold 10 mm cuvettes, 2 ball plunger screws can be adjusted
to secure an optimal reproducible cuvette position
5.
Focusing of the lens: Loosen the set screw of the collimating lens with the Allen Wrench key 1,27
mm on the barrel and slide the inner barrel until you see the optimal signal for your
spectrometer in raw data intensity counts.
The CUV-DA can be used for
fluorescence applications
as well, for fluorescence follow these
instructions:
1.
Attach the cuvette holder directly to the SMA port of the light source
2.
Move the SMA connector from the 180° position to the 90° position and screw the aluminum
mirror into the 180° position.
3.
Attach an SMA terminated optical fiber between the 90° port on the side of the cuvette holder
and the spectrometer
