IMPORTANT!
The software guides you through the calibration of each dye
separately. You must set up, run, and analyze each dye independently.
1.
In the QuantStudio
™
Real-Time PCR Software Home tab, click Instrument
Console.
2.
In the Instrument Console, select the icon for your instrument, then click
Manage Instrument.
Note:
If the Manage Instrument button is inactive, add your system to the My
Instruments group.
3.
In the Instrument Manager, start the calibration:
a.
Click Maintenance, then click Dye.
b.
In the Dye Calibration screen, select System Dye Calibration, then click
Start Calibration.
4.
In the Dye Calibration screen, select the dye to calibrate from the Dye Name
drop-down list, then perform the calibration as instructed.
5.
At the bottom of the Setup tab, enter the reagent information for the dye plate or
array card that you are using.
6.
Load the dye plate or array card into the instrument:
a.
From the instrument touchscreen, tap to eject the instrument tray.
Perform the dye
calibration
Chapter 2
Calibrate the instrument
Dye calibration
2
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QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
