3.
Edit the following fields to create a custom protocol:
• Protocol name
• Sample volume
• Stage label
Note:
A stage is made of several steps and can be iterated. You cannot iterate steps but you
can add a number of steps to a stage.
• Index (this value should an integer)
• Temperature
• Hold time
• Step identifier
• Ramp rate
4.
Issue the command from PMS (Server) to start the protocol run.
ExecuteProtocol( *requestId
[RequestID]
, *lockId
[LockID]
, *SampleVolume
0 , *RunMode "" , *CoverTemperature 0 , *Delay 0 , *Protocol
"MyRunProtocol" , *RunTitle "MyRun" );
(Optional)
View the progress of the thermal protocol run
• View the progress of the thermal protocol by subscribing to two main topics.
•
SubScribe(*requestId [
RequestID
], *lockId [
LockID
], *Event "Run");
•
SubScribe(*requestId [
RequestID
], *lockId [
LockID
], *Event
"Temperature");
Chapter 3
Operate the Automated Thermal Cycler (ATC) System as part of a robotic system
Perform a thermal protocol run
3
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Automated Thermal Cycler (ATC) System User Guide
