Program and execute a run on the ATC
3
4
1
2
7
6
8
9
10
5
Figure 10 Run Protocol
1
Add (+), Remove (−) stages
2
Remove and arrange order
3
Run Mode
drop down
4
Cover Temperature
and
Sample Volume
5
Temperature
,
Ramp Rate
,
Hold Time
6
Cancel
7
Start Run
8
Export SiLA
9
Save
10
Load
1.
In
Stages
, add or remove protocol stages with the plus and minus signs.
2.
Click up and down arrows to arrange the sequence of stages.
Specify the number of cycles required for the particular stage.
3.
In the
Steps
field, specify the temperature set points, ramp rate, and duration.
The ramp rate is indicated as a % where 100% corresponds to a peak block ramp rate of 3.5C/s.
Type in −1 in the
Hold Time
field to achieve an infinity hold time.
4.
Enter cover temperature and sample volume.
5.
Click
Start Run
to start immediately, or
Save
for future use.
Chapter 2
Operate the Automated Thermal Cycler (ATC) System as a stand-alone system
Program and execute a run on the ATC
2
18
Automated Thermal Cycler (ATC) System User Guide
