Agilent 1100 Series Fraction Collectors User’s Guide
17
Configuration and Operation of the Fraction Collector
1
Delay volumes and delay calibration
If the internal signal delay is longer than the delay time t
D1
some of the peak
will be lost. The maximum allowed signal delay time can be calculated using
the following equation:
After calculating the maximum signal delay time a
Peakwidth
setting can be
selected that gives a signal delay time, which is shorter than the calculated
maximum signal delay time. This
Peakwidth
setting should then be used for
the purification run.
Signal delay time
max
(
)
V
D
1
ν
·
----------
=
ν
·
Flow rate
=
N O T E
We recommend to set the
Peakwidth
always to > 0.01 for the DAD and MWD or to > 0.005
for the VWD
If the
Peakwidth
setting cannot be reduced and the signal delay time is longer than t
D1
it is
also possible to enhance V
D1
by adding additional tubing. However, this is not recommend
because of increasing peak dispersion caused by the higher delay volume.
The stop-time of the run in the ChemStation must be set to at least:
Total duration of time table (time of last entry
Off
) + fraction collector delay time
End of last peak (t
E
) + fraction collector delay time
V
D
1
ν
·
⁄
(
)
0.1
min
+
V
D
1
ν
·
⁄
(
)
0.1
min
+
