Verifying the installation and setup
Performing a mock run
92
AssayMAP Bravo Platform Installation Guide
2
In the
A. Application Settings
area, specify the following settings for the run:
3
Confirm that the physical layout on the AssayMAP Bravo deck matches the
Deck Layout
and
Labware Table
sections of the interface.
4
Ensure that the labware are properly seated in the platepads.
5
Click
Run Cartridge Transfer
to start the run.
To monitor the progress of the run, check the
Status
box in the upper right
corner of the form.
6
When the run is finished, remove the 96AM Cartridge Rack and Receiver
Plate from deck location 6.
Performing a mock run of the Affinity Purification protocol
IMPORTANT
For this mock run, you will not use any additional labware.
Ensure that the only items on the deck are the 96AM Wash Station at deck
location 1 and the Cartridge & Tip Seating Station containing the Resin- Free
cartridges at deck location 2.
To perform a mock run of the Affinity Purification protocol:
1
In the
Protein Sample Prep Workbench
, open the
App Library
and locate
Affinity
Purification
. Click
App
to open the application.
The Affinity Purification application opens.
Setting
Value
First Column in Source
Cartridge Rack
1
Number of Columns to Transfer
12
First Column in Destination
Cartridge Rack
1
CAUTION
Improperly seated labware can cause a hardware collision, resulting in
equipment damage. Ensure that all labware are properly seated within the alignment
features of their respective platepads.
