Pannoramic MIDI 1.16 User’s Guide
4 Software Description
Manual Focus
By selecting the
Use manual focus
box, you can preset the coarse focus value. By activating
the
Coarse focus
function, the lower limit value is set to -200, and the upper limit value is
set to +200 of the current focus value.
Note:
Before entering coarse focus value, turn live image on and select a
suitable exposure time.
You can accept the current z-value by clicking
Set Value
. This is recommended for a very flat
specimen.
If you select the
Interpolated focus
option, the coarse focus level is calculated from three
different focus points of the specimen. Carry out a manual focusing sequence in the
preview image on those three points. Set the correct focus value of the desired preview
position by clicking
Set.
If you want to go back to any of the previously set focus points, click
the appropriate
Goto
button. To delete the stored values, click
Clear Values
. The software
calculates a coarse focus level from the three values determined this way as a basis for the
fine focusing routine. You can only define the start parameter here. This will, however,
speed up the focusing process.
If you deselect the
Use fine focus
checkbox, the software immediately starts scanning on
the basis of the interpolated values, without pre-fine-focusing.
Manual Exposure
By selecting the
Use manual exposure times
, you can determine the exposure times. The
values are typically set on
Auto
, meaning that the exposure times are determined
automatically.
After selecting the corresponding filter and activating the live image, you can preset an
exposure time in the
Exposure value
field.
It is recommended to go to various positions in order to determine the intensity distribution
throughout the specimen and define a representative value. You can accept the value for
presetting by clicking
Set Value
. Repeat the procedure for all other filters.
January 16, 2013 - Rev 1
3DHISTECH Ltd.
85(127)