Version 2, May 2017
Pag. 21
Instruction manual HBB003
Neither the manufacturer nor the distributor will assume any responsibility for dama-
ges caused in the equipment, installations or injuries if user does not follow electrical
procedure. Electrical supply 220 V, 50 Hz ± 10%.
Lower the stage according to the appropriate working distance for the objective with
lesser magnification before placing the sample on it.
Put the slide on the stage and, by looking directly at it (not by the eyepiece), place the
sample centred on the stage opening.
Looking through the eyepiece using the objective with lesser magnification, raise the
stage by using the coarse adjustment knob until the image of the sample appears. Then,
move the fine adjustment knob until the image is focused.
Once the sample is focused with the objective with lesser magnification, screw the focus
limit screw
(Figure 4)
, so as avoiding the sample to hit accidentally the objectives and pro-
ducing damages on them and on the sample.
IMPORTANT: operation should always start by using
the objective with lesser magnification, as it facilitates
focusing and makes impossible to ruin the slides or get
the objectives dirty. Furthermore, it is essential for adjus-
ting the lighting previous to using the higher magnifica-
tion, and it helps to see the whole topographic structure of
the sample so as later concentrate on the more appealing
parts to observe them at higher magnifications.
By turning the nosepiece, pass to an objective with hig-
her magnification. As they are parfocal objectives it will
only be necessary to adjust focusing with the fine adjus-
tment knob.
Adjust both the diaphragm aperture until getting the light intensity desired. Normally,
when operating with an intense lighting the condenser’s numerical aperture should be
lesser than the objective’s, in order to avoid glares on the field of view that may distort the
image contrast.
Iris diaphragm enables decreasing the numerical aperture of the condenser to a value
Figura 4
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Figura 3
[1]
[2]
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Version 2, May 2017
Pag. 18
Instruction manual HBB003
These are the main features of the equipment:
Metallic body very stable with knobs for coaxial coarse and fine focusing.
Monocular head, inclined 45º and rotary 360º
- Focusing cover: Independent 30mm with final stop
- Fine 30 mm (0-200 micron each 2 microns)
Fixed condenser with simple len (A.N._0.65) with iris diaphragm and mobile filter hol-
der (blue filter Ø 32 mm included)
Lighting high intensity LED type with switch and control potentiometer of the intensity.
Optical specifications
Achromatic objectives: when talking about objectives, it is possible to distinguish bet-
ween two types. Dry objectives are the ones where there is only air between the objective
and the microscope slide, whereas immersion objectives are those that need immersion oil
to be placed between the lens and the slide so as to obtain a higher luminosity.
Zuzi microscopes serie 116 are provided with three dry objectives (4x, 10x and 40x). The
40x objective is also retractile, which means that they present a built-in mechanism to
avoid the breakage of the objective or the slide if the objective contacts the sample.
The features of objectives are inscribed on the barrel of each objective in the following
way:
40:
Magnification
0.65:
Numerical aperture (N.A.)
160:
Mechanical tube length
0.17:
Cover glass thickness
Magnification
4x
10x
20x*
40x (R)
Working distance
37.50 mm
7.32 mm
1.26 mm
0.63 mm
Numerical aperture
0.10
0.25
0.40
0.65
(R) Retractile
* Optional
Type / magnification
WF/10x
WF/16x*
Focal distance
24.99 mm
15.58 mm
Field of view diameter
18
11
* Opcional
Eyepieces: They are composed by two lenses separated by a diaphragm. Eyepieces work
in combination with microscope objectives to further magnify the intermediate image so
that sample details can be observed.
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ENGLISH
ENGLISH
