Zeiss LSM 510 Inverted, Руководство по эксплуатации

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INTRODUCTION TO LASER SCANNING MICROSCOPY
LSM 510 Performance Features of the LSM 510
B 40-051 e 07/98 3-7
3.4 Performance Features of the LSM 510
3.4.1 Optical and mechanical aspects
The highly integrated system design makes for the shortest possible optical paths, top-grade optical
precision and high stability. The compact scanning module can be fitted to an inverted (Axiovert 100 M
BP or SP) or upright (Axioplan 2
MOT
) microscope in less than three minutes. On the Axiovert, the
scanning module may be mounted either to the base port directly below the microscope or to the side
port.
The spectral range available extends from the UV to the IR region.
For the VIS (visible-light) Laser Module, the user can select from up to five lasers with wavelengths of
633, 568, 543, 514, 488 and 458 nm. The UV Laser Module provides wavelengths of 351 and 364 nm.
Coupling of the laser light is through polarization-preserving single-mode optical fibers. One variable
beam collimator each for the UV and visible ranges provides optimum adaptation of the respective laser
wavelength to the objective used and, thus, optimum correction for Z aberrations.
Acousto-optical tunable filters (AOTF) adjust the necessary brightness for all desired laser lines within
microseconds.
A monitor diode permanently registers the laser output; it can be used for the on-line checking of the
intensity of the exciting light. This check is also possible selectively for the different wavelengths if a line
selection filter is inserted.
The four simultaneous image acquisition channels, usable for reflection or fluorescence, and an
additional transmitted-light channel are ideal for the investigation of multiple fluorescence specimens.
Separately in each of the four channels, the diameters of the pinholes and their XY positions can be
optimized, and the desired emission filter placed into the beam path, by servo-motor control. In the case
of pinhole VP1, this adjustment also includes positioning along Z. In the simultaneous registration of
multiple fluorescences, identical optical sections can be obtained in each confocal channel. This is of
importance, e.g., with the FISH method (fluorescence in-situ hybridization) used for genome analysis in
cytogenetic studies.
The microscope's transmitted-light channel is equipped with a photomultiplier, too. It is therefore
possible to superimpose a multiple fluorescence image on a brightfield, differential interference or phase
image.
A fiber-optic cable connection to external special detectors, such as cooled PMTs or spectrometers, is
under development.
In addition to the emission filters for all standard and special applications, available in motor-controlled
filter wheels, the user can easily install his own emission filters in two of the channels.
The high-NA C-APOCHROMAT objectives specially developed for the LSM technique reach the physical
limit in resolving power, and can be used throughout the 350...700 nm spectral range with the same
high quality, producing brilliant images.
A two-mirror scanner system, controlled by a digital signal processor (DSP), offers several advantages.
The large deflection angle of the scanning mirrors allows a wide area to be scanned. With a 1.25x
objective, the object area scanned is 10 x 10 mm².