Zeiss LSM 510 Inverted, Руководство по эксплуатации

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OPERATION
LSM 510 Annex
B 40-051 e 07/98 4-177
Clicking on the additional <Leveling> button moves the HRZ 200 to the zero position, while the
motor focus moves into the opposite direction at the same time, i.e. the position of the object in
relation to the objective remains unchanged. This function is used to set defined initial conditions.
The <Calibration> slider must normally be left in the default position 0. Calibration is required only if
the examined image field is located clearly outside the center of the specimen carrier on the HRZ 200.
Calibration is described in the next section.
4.12.4.4 Additional information on the operation
The HRZ 200 fine-focusing stage is a high-precision, sensitive accessory for the LSM 510 from Carl Zeiss
and must therefore be treated carefully. High mechanical stress, such as the use of specimens weighing
more than 100 g or the application of pressure or knocks on the movable stage tongue, can result in
damage and therefore in failure of the stage function.
To be able to fully utilize the outstanding precision attainable with the fine focusing stage, anything
which could interfere with its operation, especially mechanical knocks and impact of the LSM
components, should be avoided. We would recommend you to always use the actively vibration-damped
Kinetics stage (available as accessory under the order number 1007 508 or 1007 512) as the base for the
setup of LSM systems containing the HRZ 200 stage.
The specifications of the stage are obtained only after a heating phase of approx. 30 minutes.
Furthermore, the installation conditions for the LSM system must be observed.
The maximum reproducibility (better than 40 nm) for moving to an absolute position in z is achieved by
always moving to the required position from below.
Fine focusing is performed mechanically via an inclined position of the stage tongue. Therefore, the
lifting range z at the location of the image field depends on the position of the HRZ in relation to the
optical axis. This means: if the user shifts the object on the microscope stage to the right via the HRZ
200, the lift will be different from the one in the zero position of the stage (max. 200 µm) and also from
the one after a shift of the stage to the left.
If the LSM system is equipped with a motorized scanning stage, this shift is read back to x and the lift is
calibrated automatically if the zero position of the HRZ has been matched to the zero position of the
scanning stage via an initialization run. For this, activate the <Stage> dialog in the <Acquire> menu.
Then position the scanning stage in such a way that the optical axis of the microscope corresponds to
the zero position of the HRZ, i.e. to the center of the specimen holder in the stage tongue. Then perform
initialization by pressing the <HRZ Null> button. This step must be repeated after every new start of the
system. Also see the notes on the operation of the motorized scanning stages.
If the system is equipped with a manual microscope stage, the user has the option of performing the
calibration by entering the
x shift in mm via the <Calibration> slider.