OPERATION
Annex
LSM 510
4-178
B 40-051 e 07/98
The shift is read off from the microscope stages. In the case of the manual Axioplan 2 stage,
x can be
read directly from the scale adhered to the front of the stage. In the case of the manual Axiovert 100
stage, a scale is located on the right of the knob, where the 45 mm
x shift relative to the zero position
of the microscope stage can be read off. The
x value is positive for both stages if shift from the zero
position is made to the right and negative if the shift is made to the left.
On account of the inclined position of the stage tongue, the object is also shifted laterally during the fine
focusing motion. This lateral shift is negligibly small if, as recommended by us, specimen carriers with
thickness 1.0 mm are used exclusively. Otherwise, the marked lateral shift of the object during fine
focusing can result in image distortion. For the same reason, Petri dishes without fixation ring must be
used exclusively.
The nosepiece of the Axiovert stand is moved to the load position prior to switching off the LSM system
and the HRZ 200 is then moved to the lowest position to avoid damage of the objective or object by a
possible collision. The user must refocus after start-up of the system. Before an objective change in the
Axiovert or the Axioplan, the nosepiece and the microscope stage must be moved to the Load Position
by the user, and then back to the Work Position to prevent the objectives from hitting the HRZ
components. This is performed automatically if the objectives are changed menu-controlled via the
relevant buttons of the LSM program.
The HRZ 200 for the Axiovert 100 M (1013 186) or for the Axioplan 2
MOT
(1013 187) can be attached
to the following standard microscope stages:
mechanical stage 85 x 130 for Axiovert (45 13 39)
scanning stage DC 100 x 90 for Axiovert (45 17 40)
mechanical stages 75 x 50 for Axioplan (45 35 05, 45 35 02-99 04, 45 35 07)
scanning stage DC 4“ x 4“ for Axioplan (45 35 85-99 01)
In the case of the last configuration, the object plane is shifted upwards so that Köhler illumination and
classical transmitted-light microscopy will no longer be possible because the condenser cannot be moved
sufficiently close to the object.
The user will not have to deal with any other restrictions.
Содержание LSM 510 Inverted
Страница 1: ...I LSM 510 Laser Scanning Microscope Operating Manual...
Страница 4: ...INTRODUCTION LSM 510 IV B 40 051 e 07 98...
Страница 10: ...INTRODUCTION LSM 510 X B 40 051 e 07 98...
Страница 12: ...NOTES ON DEVICE SAFETY Contents LSM 510 1 2 B 40 051 e 07 98...
Страница 22: ...NOTES ON DEVICE SAFETY Warning and information labels LSM 510 1 12 B 40 051 e 07 98...
Страница 24: ...LSM 510 SETUP REQUIREMENTS Contents LSM 510 2 2 B 40 051 e 07 98...
Страница 34: ...LSM 510 SETUP REQUIREMENTS Laser Module VIS Laser Module UV LSM 510 2 12 B 40 051 e 07 98...
Страница 36: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Страница 46: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Performance Features of the LSM 510 LSM 510 3 12 B 40 051 e 07 98...
Страница 48: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 3 2 B 40 051 e 07 98...
Страница 58: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Performance Features of the LSM 510 LSM 510 3 12 B 40 051 e 07 98...
Страница 231: ...OPERATION LSM 510 Annex B 40 051 e 07 98 4 173 Fig 4 171 Change over of the Scanning Module...
Страница 256: ...INTRODUCTION TO LASER SCANNING MICROSCOPY Contents LSM 510 5 2 B 40 051 e 07 98...
