OPERATION
Carl Zeiss
Illumination and Contrast Techniques in Transmitted Light
Axio Vert.A1
82
431030-7044-001
05/2012
4.11.5
Transmitted Light PlasDIC
PlasDIC, an innovative interference contrast, delivers relief-like images and is to be used for thicker
specimens, in particular.
The contrast is variably adjustable. The cavities of microtiter plates can be contrasted right to the edge.
Cultivation vessels are not required, but are equally suitable.
Requirements
−
The microscope must have properly been put into operation, as described in Section 3.
−
The microscope must be switched on.
−
Condenser with slider for PlasDIC or condenser 0.4 or 0.55 with modulator disk and built-in slit
diaphragm for PlasDIC
−
PlasDIC slider, dependent on the objective used, for the corresponding objective position in the
nosepiece
or
three-position contrast slider (10 mm x 29 mm) with built-in LD A-Plan 10x-63x PlasDIC module for
the contrast slider (suitable for LD A-Plan 10x to 63x objectives)
−
D P&C analyzer module in the reflector turret or three-position contrast slider with built-in analyzer for
the contrast slider (one of the two required if a PlasDIC slider is used)
−
Objective for PlasDIC (e.g., LD A-Plan 10x, 20x, 32x, 40x or 63x)
Setting PlasDIC
•
Open the aperture diaphragm on the condenser completely. Put on the specimen.
•
Rotate in the PlasDIC diaphragm on the condenser (Fig. 4-8/
1
) with modulator disk (or move the
PlasDIC position of the slider on the condenser into the light path). Brightness may have to be
increased, as appropriate, when you change from bright field to PlasDIC.
•
When employing a PlasDIC slider, rotate the D P&C analyzer module of the reflector turret into the
light path or move the three-position contrast slider with the analyzer position (Fig. 4-8/
2
) into the slot
under the nosepiece. If you use the PlasDIC module with the three-position contrast slider, no analyzer
will be required.
•
Rotate in the objective for PlasDIC. The following are suitable objectives: LD A-Plan 10x, 20x, 32x, 40x
or 63x, LD Plan-Neofluar Korr 20x, 40x or 63x.
•
On the nosepiece, place the PlasDIC slider (Fig. 4-8/
4
) into the DIC slot of the objective used or move
the PlasDIC module, sitting in the slider, into the light path.
•
Set the contrast via the knurled thumb screw of the PlasDIC slider (Fig. 4-8/
5
) or of the PlasDIC
module. Structures may be shown in relief or in pseudo dark field images. Relief imaging delivers the
best contrast.
Содержание Axio Vert.A1
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