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UltraMicroPump II

World Precision instruments

References

S.B. Mazzone, D.P. Geraghty

“respiratory actions of tachykinins in the nucleus of

the solitary tract: effect of neonatal capsaicin pretreatment” (2000)

British Journal

of Pharmacology

129

:6 pp1132-1139.

B.L. Davidson, C.S. Stein, J.A. Heth, I. Martins, R.M. Kotin, T.A. Derksen,
J. Zabner, A. Ghodsi, J.A. Chiorini

“recombinant adeno-associated virus type 2,

4, and 5 vectors: transduct ion of variant cell types and regions in the mammalian
central nervous system” (2000)

Proceedings of the National Academy of Sciences

of the United States of America

:7 pp3428-3432.

A.I. Brooks, et al.

“reproduciable and efficient murine cns Gene delivery using

a microprocessor controlled injector” (1998)

Journal of Neuroscience Methods

pp 137-147.

Содержание UltraMicroPump II

Страница 1: ...UltraMicroPump II UMP2 Microsyringe Injector and Micro4 Controller INSTRUCTION MANUAL Serial No _____________________ 071807 World Precision Instruments Inc ...

Страница 2: ...m without prior written permission of World Precision Instruments Inc Contents Introduction 1 Parts List 2 Set up and Operation 2 Setting Parameters 6 UltraMicroPump II Operation 13 Defining Other Syringes 14 Computer Control 18 General Maintenance 25 Troubleshooting 25 Specifications 22 Syringes 23 Accessories Replacement Parts 24 References 27 Appendix Nanoliter 2000 Micro4 Volume Settings 28 Wa...

Страница 3: ...traMicroPump accepts syringes from 0 5 µL to 250 µL UltraMicroPump can be useful for a wide range of applications including intracellular injection micro delivery of biochemical agents or dyes cell separation in vitro fertilization and can be mounted directly onto a stereotaxic frame or micromanipulator Fig 1 UltraMicroPump II and Micro4 controller The pump above is mounted on WPI s M3301 microman...

Страница 4: ... Supply Power Cable Allen key Instruction Manual For a list of microsyringes available from WPI see page 23 Set up and Operation These instructions will help you put the UltraMicroPump to immediate use We recommend that you read the entire manual and familiarize yourself with the various operating procedures of the UltraMicroPump and Micro4 Mounting the syringe Syringes may be filled manually befo...

Страница 5: ...below and behind the collar stop in the groove where the long drive screw is there is a small Phillips head screw attached to the collar stop Loosen this screw to slightly about 0 5 to 1 mm to allow for a thicker collar see Fig 2 You may have to grasp the collar stop and wiggle it backwards to move it Once the stop is backed out check and adjust for a tight fit so the syringe body doesn t move whe...

Страница 6: ...ith Stoelting frames Connect the controller Plug the UltraMicroPump cable into an output socket on the back of the Micro4 controller see Fig 3 Up to four pumps may be connected and independently controlled Plug in the 12 volt power supply and power up Plug the 12 volt power supply into the rear panel of the Micro4 see Fig 3 The switchable power supply included with this unit automatically senses i...

Страница 7: ...ideration to surface tension heat pressure compressibility silanization or air bubbles the 3 error rate should yield a 300 nL variance As a rule of thumb the choice of the syringe should not exceed 1 10th to 1 20th the stated full volume of the syringe The Micro4 UMP2 system uses a stepper motor to move the syringe piston forward to inject the volume A consideration should also be made to allow th...

Страница 8: ...rouped Non grouped and Disabled Run Stop Starts and stops the pump s Data Save Saves all parameters on LCD display to memory Next time the Ultra Microsyringe Pump controller is turned on the last paramaters saved will be displayed Lights When a pump is running the correspond ing light is on For example the light next to output 1 will be on when pump one is operating Fig 4 Micro4 front panel Settin...

Страница 9: ...bled when the cursor is in the Infuse Withdraw field position the cursor in the Infuse Withdraw field and press the corresponding option number Disables audible tone Enables audible tone Changes the action of run stop key or footswitch When this option is enabled the pump will operate as long as the run stop key or footswitch is pressed and stop when the run stop key or footswitch is released Retu...

Страница 10: ...tion Accuracy Since every syringe in the microliter volume range has its own unique intricacies you should verify and calibrate each syringe and log its characteristics for accurate injections The Micro4 controller has preset types of syringes to very accurately move the plunger button of the syringe a precise distance per injection VOLUME SET nL Volume Counter Real time display of volume being de...

Страница 11: ...n in a 500 µL syringe 4 Multiply times 60 mm for the actual distance travel 0 3 60 18 mm 60 mm is the full volume stroke of the syringe 5 Check 75 000 250 000 0 3 0 3 60 mm 18 mm 6 Any combination of syringe types and volume choices can be used to displace a particular length as long as the volume is noted and the injection speed does not exceed the rated value nearest that syringe volume See syri...

Страница 12: ...select key to choose either S nL sec or M nL min S nl sec M nl min DEVICE TYPE Device syringe Type The volume per step and rate data for eleven microsyringes are already stored in Micro4 s memory To specify one of these syringes position the cursor in this field and use the select key to change the syringe type to a letter A L correspond ing to that of the syringe in the table below ...

Страница 13: ...I in these volumes instead use a liquid tight syringe to prevent drive motor damage The ILS005 5 µL syringe must use type M with a 0 5880 nL step entry See page 15 for details These are defined as 60 mm in Rev H firmware WPI s Nanoliter 2000 a nanoliter injector for the 2 70 nL range comes with its own simple controller but may also be driven by the Micro4 For more information enquire about WPI B2...

Страница 14: ...s pressed while the cursor is located on any grouped channel Non grouped mode When the cursor is positioned on a channel that is not grouped indicated by the letter N only that channel will start or stop when the run stop key is pressed Disabled mode When a channel is disabled the line of data is hidden and the pump will not operate No changes may be made to this channel while it is disabled To re...

Страница 15: ...crements as an indication of the plunger s motion Multiple injections can be achieved by pressing the run stop button again after the pump has stopped Fast forward Press and hold the right arrow key then press run stop The syringe pump will continue running as long as these two keys are depressed or until the set volume is reached Fast reverse Press and hold the left arrow key then press run stop ...

Страница 16: ...yringe of known ID Volume per Step ID 2 x ID 2 x 3 1415926 x 1000 x 0 003175 Syringe of unknown ID The Inside Diameter ID of a syringe can be determined by this formula ID SQRT Volume 3 14159 x Length x 2 where length is the visible marking on the syringe body total length in mm and Volume is the full volume of the syringe in microliters For example if you have a 60 mm syringe with a 2 5 µL volume...

Страница 17: ...ibrated see page 17 for details Solution 2 Using Type M divide the Type C nL step number by 0 4990 Example The standard 54 1 mm syringe has a value of 0 2934 nL step on a 5 µL syringe The ILS005 has a 27mm stroke for this same full volume 0 2934 0 4990 0 5880 is the nL step value to enter following the Type M instructions on this page 0 5880 is also verified by the formulas on the previous page Af...

Страница 18: ...e syringe and the stiffness goes away The stiffness of the new plunger tip may require you to move the rod in small increments until the tip is formed enough to actuate by the rods full length Wet Autoclaving Syringes Typically the answer to autoclaving any Teflon syringe is no since the adhesives and the Teflon seal will eventually breakdown or swell from the heat and pressures involved The most ...

Страница 19: ...alibration Volumetric diameter measurement Using a microscope and a calibrated reticle or stage micrometer inject an amount of water into a hydrated oil droplet measure the sphere and use Volume Diam 2 Diam 2 Diam 2 3 1415926 4 3 1000 to determine the volume in nanoliters Diameter in mm Analytical Balance Use an analytical balance to weigh the mass of the injected volume Pure water is 1 gm 1 mL at...

Страница 20: ...must have a decimal point and terminate with semicolon see page 19 R Sets the delivery rate Number must have a decimal point and terminate with semicolon see page 19 I Infuse mode W Withdraw mode G Go Starts the syringe pump H Halt Stops the syringe pump S Sets the rate units to nanoliters second M Sets the rate units to nanoliters minute L Line number sets the syringe number on display Micro4 onl...

Страница 21: ... see a response on the UMC4 display from each entry as it is entered The cursor channel indicator the L command must be on the pump channel that is being run If the cursor is elsewhere then the command will not function on other channels unless the grouping is turned on G last column Format of the V volume command The character field of V is a maximum of five numerals a 6th character can be the pe...

Страница 22: ... 2035 maximum rate R1000 1 1000 V120 will yield 12000 since the decimal point was not entered Display not showing programmed values If the volume and the rate cannot immediately be seen on the Micro4 display this could be due to the Grouping being turned off the programmed units display comes up immediately on acceptace of the transmission The first item to test is the line number command and see ...

Страница 23: ...nter specify the C parameter as C0 0 with the Volume and Rate on a separate command line just before issuing the G go command RS232 Cable Pinouts To control the Micro4 by computer the RS232 cable must be configured as shown here Footswitch Connections Since the footswitch produces Run and Stop signals by connecting 5 volts from pin 4 to pin 6 this port may also be used for TTL signals from other s...

Страница 24: ... motor Please use liquid tight syringes for applications that require volumes greater than 250 µL Troubleshooting Problem The instrument motor makes noise but the plunger button does not travel Check Look for a loose connector at the rear of the Micro4 make sure the UMPII plug is firmly seated The gray plastic plug should be a flush fit with the connector on the controller Verify that the pins in ...

Страница 25: ...is syringe type requires more force than the motor can push If you require a large volume syringe over 250 µL please use a liquid tight plunger 2 Needle blockage The micropipette or the needle might be blocked by a tissue mass in or outside of the needle or the needle tip may be too small for the programmed injection Check for normal operation of the pump in air with and without the syringe attach...

Страница 26: ...osition Error 0 5 Pump Force 400 g Syringe Diameters 5 5 mm to 9 0 mm Maximum Step Rate 700 steps sec depending on syringe Weight 325 grams 11 4 oz Size Ø 32 mm x 190 mm Ø 1 3 in x 7 5 in Power Requirements 12 VDC 2 amps provided by Micro4 Micro4 Controller Power Requirements 12 V 1 6 A Dimensions 12 7 x 15 2 x 8 9 cm 5 x 6 x 3 5 in Power Requirements 12 VDC from auto switchable power supply 100 2...

Страница 27: ...N 23 ga 0 63 mm 70 mm long RN001 For Syringe SGE001RN 26 ga 0 47 mm cone 70 mm long RN010 For Syringe SGE010RN 26 ga 0 63 mm 50 mm long 5 pack RN025 For Syringe SGE025RN 25 ga 0 63 mm 50 mm long 5 pack RN025 For Syringe SGE050RN 25 ga 0 63 mm 50 mm long 5 pack RN025 For Syringe SGE100RN 25 ga 0 63 mm 50 mm long 5 pack Syringes with luer fitting no needle ILS005LT 5 µL Gas tight Microsyringe Glass ...

Страница 28: ... II 300033 Adaptor for Micro4 to Nanoliter 2000 B203MC4 Nanoliter 2000 and Micro4 controller B203XVY Nanoliter 2000 Injector USA 110V power adapter included Replacement parts 65134 Mounting Bar 65085 Mounting Bar Locking Nut 65015 Collar Stop Nut For special connections 6 pin Miniature DIN plug Digi Key CP 20600 ND Not available from WPI ...

Страница 29: ...A Heth I Martins R M Kotin T A Derksen J Zabner A Ghodsi J A Chiorini Recombinant adeno associated virus type 2 4 and 5 vectors Transduct ion of variant cell types and regions in the mammalian central nervous system 2000 Proceedings of the National Academy of Sciences of the United States of America 97 7 pp3428 3432 A I Brooks et al Reproduciable and Efficient Murine CNS Gene Delivery Using a Micr...

Страница 30: ...o4 panel will be calculated as 100 2 3 or 43 47 steps The motor can only step in whole numbers so the volume must be adjusted up or down to the nearest whole step value Increasing to 44 steps times 2 3 gives a volume of 101 2 nL decreasing to 43 steps times 2 3 gives a volume of 98 9 nL One of these two volumes should be used to insure a proper injection Leaving the value on the Micro4 at 100 nL w...

Страница 31: ...erate in the manner desired by the user WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product Claims and Returns Inspect all shipments upon receipt Missing cartons or obvious damage to cartons should be noted on the delivery receipt before signing Concealed loss or damage should be reported at once to the carrier and an inspecti...

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