C-16 Solvent Considerations
Acids and bases
Acetic acid,
1%
2.61
2.63
2.61
2.43
2.17
0.87
0.14
0.01
<0.01 —
Hydro-
chloric acid,
0.1%
0.11
0.02
<0.01 —
—
—
—
—
—
—
Phosphoric
acid, 0.1%
<0.01 —
—
—
—
—
—
—
—
—
Trifluoro-
acetic acid
1.20
0.78
0.54
0.34
0.22
0.06
<0.02 <0.01 —
—
Diammo-
nium
phosphate,
50 mM
1.85
0.67
0.15
0.02
<0.01 —
—
—
—
—
Triethy-
lamine, 1%
2.33
2.42
2.50
2.45
2.37
1.96
0.50
0.12
0.04
<0.01
Buffers and Salts
Ammonium
acetate,
10 mM
1.88
0.94
0.53
0.29
0.15
0.02
<0.01 —
—
—
Ammonium
bicarbonate,
10 mM
0.41
0.10
0.01
<0.01 —
—
—
—
—
—
EDTA,
disodium,
1 mM
0.11
0.07
0.06
0.04
0.03
0.03
0.02
0.02
0.02
0.02
HEPES,
10 mM,
pH 7.6
2.45
2.50
2.37
2.08
1.50
0.29
0.03
<0.01 —
—
MES,
10 mM,
pH 6.0
2.42
2.38
1.89
0.90
0.45
0.06
<0.01 —
—
—
Mobile phase absorbance measured against air or water (Continued)
Absorbance at specified wavelength (nm)
200
205
210
215
220
230
240
250
260
280
Содержание 2998
Страница 12: ...xii Table of Contents...
Страница 26: ...1 14 2998 PDA Detector Optics Principles...
Страница 62: ...3 12 Maintaining the Detector...
Страница 82: ...5 10 Spectral Contrast Theory...
Страница 96: ...A 14 Safety Advisories...