Maintaining the 1525 Pump
67
4
8.
Holding the pump head securely against the pump, reinstall the two pump head
mounting screws. Alternately tighten each screw, using half turns to evenly align the
assembly.
9.
Using the software, navigate to the Inlet Method window. Set the Flow parameter to
0.3
mL/min, click
Save
, then click
Load Method
to start the pump. Verify that the
indicator rod moves freely.
10. Click the
Stop Flow
button to stop the pump.
11. Use the 1/2-inch adjustable wrench to hold the check valve housings in place.
Reconnect the inlet and outlet tubings to the pump head. Tighten the tubings using
the 5/16-inch open-end wrench.
12. Prime the pump as described in
. If you notice leaks, verify
pump head and plunger seal installation.
4.5.3 Cleaning and Replacing 1525EF Plungers
The plungers are sapphire rods that require careful handling. Although cleaning the
plungers is not difficult, it is important to follow these instructions carefully to avoid
damaging the plungers. Assemble all materials and read the procedure thoroughly before
you begin.
Required Materials
•
5/16-inch open-end wrench (in Startup Kit)
•
5/32-inch Allen wrench (in Startup Kit)
•
9/64-inch Allen wrench (in Startup Kit)
•
1/2-inch adjustable wrench (in Startup Kit)
•
Snap-ring pliers (part number WAT025263)
•
Seal extraction tool and seal insertion tool (see
for part numbers)
•
Plunger insertion tool (part number WAT011042)
•
Sonicator
•
HPLC-grade methanol
•
Replacement plunger
•
Replacement plunger seals
Removing the Plunger
1.
Remove the pump head as described in
Section 4.5.1, Preparing for Plunger Seal
Caution:
To avoid possible eye injury or cuts, handle the plunger with care. Wear safety
glasses and use the plunger insertion tool. Pieces of a broken plunger are very sharp.
