Preparing the Pump
48
3
3.2.1 Priming the Pump
Priming the pump is necessary to ensure proper pump operation when:
•
Starting the pump for the first time
•
Changing eluent
•
The system has been idle for some time
Required Material
Priming syringe (part number WAT010337 in Startup Kit)
Procedure
1.
Power on the pump.
2.
Power on the MassLynx workstation and log on to Windows.
3.
Select
Start > Programs > MassLynx
>
MassLynx V4.0
. The MassLynx Main
window appears.
4.
Click
Status
to get the LC status.
5.
Click the
Inlet Method
button (left side of the window) to open the Inlet Editor
window.
6.
Click the
Inlet
icon to go to the pump method.
7.
Set the flow rate:
•
For the 1525
µ
Pump
,
set the rate to
1.0
mL/min.
•
For the 1525EF Pump, set the rate to
3.0
mL/min.
If the pump is dry, start with a lower flow rate.
8.
Click
Save
in the Inlet Method window to save the method.
9.
Fully depress the priming syringe plunger to remove all air from the syringe, then
insert the syringe into the Luer fitting at the center of the draw-off valve handle
(
STOP
Attention:
Do not start the pump at this point in the procedure.
