Maintaining the 1525 Pump
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4.5.1 Preparing for Plunger Seal Replacement
Required Materials
•
5/16-inch open-end wrench (in Startup Kit)
•
5/32-inch Allen wrench (in Startup Kit)
•
1/2-inch adjustable wrench (in Startup Kit)
•
Priming syringe (in Startup Kit)
•
Fitting plug (in Startup Kit)
•
Replacement seal kit (in Startup Kit)
•
Plunger insertion tool (part number WAT011042)
•
Seal extraction tool (see
for part number)
•
HPLC-grade methanol
To access a plunger seal, first remove the pump head.
To remove the pump head:
1.
Purge the pump with methanol. If methanol is not miscible with your current eluent,
use an intermediate eluent.
2.
Insert the priming syringe into the Luer fitting at the center of the draw-off valve
handle, then turn the handle counterclockwise about 1/2-turn to open the valve.
3.
Use the syringe to withdraw all methanol.
4.
Use the software to navigate to the Inlet Method window. Set the Flow parameter to
0.3
mL/min, click
Save
, then click
Load Method
to run the pump.
5.
After the indicator rod fully retracts into the pump head, click the
Stop Flow
button
to turn off the pump. This ensures that the weight of the pump head does not rest on
the plunger while you remove the head.
6.
Use the 1/2-inch adjustable wrench to hold the check valve housings in place. Use
the 5/16-inch open-end wrench to remove the inlet and outlet tubings from the check
valve housings on the pump.
7.
Holding the pump head against the pump, use the 5/32-inch Allen wrench to remove
the two pump head assembly mounting screws. Loosen the screws 1/2-turn at a
time for the first two turns (
STOP
Attention:
Before starting this procedure, move the eluent reservoir below the pump
head level. This eliminates gravity flow of the eluent.
