Preparing for Operation
47
3
Priming and equilibrating procedures are described in
.
Eluent Guidelines
When preparing a 1525µ or 1525EF Pump for operation, follow these guidelines:
•
Use methanol to prime a new pump, or one that has been stored dry for an extended
period.
•
Use an intermediate solvent when changing between eluents that are not miscible.
•
Use only HPLC-grade eluents to obtain accurate, reproducible chromatography.
•
To prevent air from being drawn into the pump, keep the eluent level in the reservoir at
least 4 inches (10 cm) higher than the inlet manifold. Do not place eluent reservoirs
on top of the pump.
•
Use only filtered, degassed eluents.
•
After use with a buffer, purge the pump with HPLC-grade water.
•
When possible, use eluents of similar viscosity when you run a gradient.
•
Know the properties of your eluents; for example, cyclohexane solidifies at 3.5 K psi.
•
Consider column chemistry. If the column can be damaged by an intermediate or new
eluent, remove the column from the system and substitute a union. Refer to the care
and use guide for your column.
•
Dedicate an eluent supply tube and filter assembly for each eluent. If this is not
possible, purge the tube and filter of any remaining eluent before you use a new
eluent.
•
To prevent cross contamination of eluents, dedicate a set of glassware for preparing,
storing and supplying each eluent. After a container is used for an eluent, do not use
it for any other eluent unless it has been thoroughly cleaned.
Table 3-1 Pump Preparation Recommendations
Status
Prime
Equilibrate
Initiating operation
Changing eluent
Adding fresh eluent
System has been idle for some time
Caution:
To avoid chemical hazards, observe safe laboratory practices when handling
eluents. Refer to the Material Safety Data Sheets for the eluents in use. For more
information on eluent properties, see
