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Trace2o Ltd

Rev1.0

APPLICATION NOTE

T2O-AN-P270

NITRITE (P270) METHOD

The following application note explains the procedure for the detection of Nitrite (P270) using the
HT1000 Photometer.

Equipment:



HT1000 Photometer



10ml vial



Stirring rod



Nitrite LR tablet

Safety:



Consult the safety data sheet for all of the reagents before use. Even if you have used
HydroTest reagents before, the formulation may have changed.

Getting started:



Switch the unit on using the power key.



Select the Nitrite method by depressing the cursor keys until ‘Nitrite T ’ is displayed or use
the shortcut by pressing & holding shift followed by 270

Release shift and press enter.

HT1000

Содержание HydroCheck HC1000

Страница 1: ...HydroCheck HC1000 Multi Parameter Electrochemical Water Tester Instruction Manual...

Страница 2: ...Salinity Measurement 12 3 6 Dissolved Oxygen Measurement 12 3 6 Hold Function 14 3 7 Storing and Recalling Data from Memory 14 4 1 Setup Menu 15 4 2 Adjusting the settings 20 4 3 Exiting setup mode 2...

Страница 3: ...ration in mg L or ppm Mode 34 9 2 2 Point Calibration 34 10 1 Temperature Calibration 36 11 1 pH Electrode Care and Maintenance 37 11 2 ORP Electrode Care and Maintenance 38 11 3 Conductivity Electrod...

Страница 4: ...t work environment meet following conditions Relative humidity is less than 80 Ambient temperature is greater than 0o C and less than 60o C No potential electromagnetic interference The following list...

Страница 5: ...splay that shows measured values as well as mode and status indicator graphics INDEX Measurement mode Battery indicator Calibration mode Stable indicator Setup mode Hold indicator Memory mode Calibrat...

Страница 6: ...modes pH mV ORP Ion Conductivity TDS Salinity Resistivity DO Press and hold to enter temperature setting mode cal Press to enter the calibration mode Press and hold to enter the setup menu m Press to...

Страница 7: ...des Waterproof 6 pin connector Dissolved oxygen electrode Waterproof 3 pin connector Conductivity electrode 2 4 Connecting the Sensors Take out the sensor from the carrying case Ensure the connectors...

Страница 8: ...are aligned Once pushed firmly into place tighten the waterproof locking collar DISSOLVED OXYGEN ELECTRODE Insert the 6 pin connector on the temperature probe into the corresponding connector on the...

Страница 9: ...ttery cover from the rear of the meter 2 Disconnect the snap connector and remove the battery 3 Note the polarity and attach the snap connector to the appropriate terminals on the new battery 4 Ensure...

Страница 10: ...the membrane cap from the bottom of the dissolved oxygen probe 2 Fill the membrane cap halfway with electrolyte solution 3 Screw the membrane cap onto the probe excess electrolyte will drain out 4 Be...

Страница 11: ...sample solution and stir the solution gently 4 Wait for the reading to stabilise and record the value shown on the display 3 3 ORP Measurement The HC1000 has two millivolt measurement modes ABSOLUTE M...

Страница 12: ...r and measurement unit ppt the meter enters seawater measurement mode 3 Select the desired measurement mode Immerse the electrode in the sample solution Stir the solution gently 4 Wait for the reading...

Страница 13: ...3 If necessary to set the atmospheric pressure and salinity coefficient in the setup menu Refer to SETUP MENU section 4 Submerse probe in the sample solution make sure the temperature sensor on the p...

Страница 14: ...The HC1000 allows up to 500 data sets to be stored and recalled MEMORY INPUT During the measurement process press the M key to store the measured value to the memory The Memory indicator appears on th...

Страница 15: ...NS DESCRIPTION DEFAULT Select from common pH buffer standard groups for calibration USA 1 68 4 01 7 00 10 01 12 45 NIST 1 68 4 01 6 96 9 18 12 45 DIN 1 09 4 65 6 79 9 23 12 75 User Defined Buffer Sele...

Страница 16: ...e number of calibration points to use when calibrating the meter 1 point 2 points 3 points 4 points 5 points Pure water coefficient will be calculated and applied automatically for ultra pure water me...

Страница 17: ...ric pressure coefficient Setting Range 450 to 850mmHg 760 Select the salinity coefficient of sample solution Setting Range 0 0 to 50 0ppt 0 0 Set the resolution for DO measurement 0 01mg L 0 1 0 1mg L...

Страница 18: ...rate When the HI option is enabled measuring value will stabilize slowly but improves accuracy Low High When the auto hold function is enabled the meter will automatically sense a stable end point rea...

Страница 19: ...he meter will automatically show indicator 1 to 31 days Disable Sets the date and time of the meter Clear all stored data Enable Disable Reset function allows user to restore the meter to factory defa...

Страница 20: ...gh the menu and select the parameter to amend Refer to Setup Menu section 4 Press ENTER key to confirm the display shows the currently selected option 5 Press or key to select the desired option 6 Pre...

Страница 21: ...he display shows the DATE option 3 Press ENTER key to confirm the meter shows the currently programmed year 4 Press or key to set the year 5 Press ENTER key to confirm the meter shows the currently pr...

Страница 22: ...will show on the display the meter is now switched to automatic temperature compensation mode 4 7 Manual Temperature Compensation 4 7 1 DO NOT connect the temperature probe to meter 4 7 2 Press and ho...

Страница 23: ...re that the meter is calibrated when attaching a new electrode or before first use DO NOT use visibly dirty calibration solution contaminants in solution will affect the calibration and eventually the...

Страница 24: ...eps from 5 3 3 until the display shows END Once calibration is complete the meter returns to measurement mode automatically 5 4 pH Calibration with User Defined Buffers 5 4 1 Ensure that the USER opti...

Страница 25: ...ration result does not meet measurement requirements the indicator will disappear on the display 5 5 pH Calibration Report The meter allows checking of the calibration data of the pH electrode for dia...

Страница 26: ...urrent measured value 4 Press or key to set the displayed value press ENTER key to confirm The Calibration indicator begins flashing 5 Wait for the measured value to stabilise the display shows END On...

Страница 27: ...27 4 Press key the meter shows OFS Offset Potential 5 Press the ON OFF key to return to measurement mode MR...

Страница 28: ...r to calibrate the meter When the calibration is completed all new calibration values will automatically override existing data The following table shows acceptable conductivity range of calibration s...

Страница 29: ...AL2 The meter prompts you to continue with the second calibration point 7 3 3 Rinse the conductivity electrode with distilled water Immerse the electrode in the next calibration solution the meter aut...

Страница 30: ...for 3 seconds to enter setup menu the display shows CELL option 7 4 3 Press ENTER key to confirm the meter enters the cell constant setting mode 7 4 4 Press or key to select USER option 7 4 5 Press EN...

Страница 31: ...oses 1 Press the MR key in the conductivity measurement mode the display shows LOC P 01 2 Press or key until the meter shows ELE P 02 Electrode Diagnosis 3 Press ENTER key to confirm the meter shows t...

Страница 32: ...ement unit 8 2 2 Make sure that 1 point calibration is selected in the setup menu 8 2 3 Press CAL key the meter enters calibration mode the display shows 100 CAL1 8 2 4 Hold the dissolved oxygen probe...

Страница 33: ...oxygen probe in air saturated water for 3 to 5 minutes press ENTER key to confirm Wait for the measured value to stabilise the display shows END Once calibration is complete the meter returns to meas...

Страница 34: ...r saturated water for 3 to 5 minutes 9 1 6 Press ENTER key to confirm Calibration indicator begins flashing 9 1 7 Wait for the measured value to stabilise the display shows END Once calibration is com...

Страница 35: ...9 2 7 Immerse the dissolved oxygen probe in air saturated water for 3 to 5 minutes press ENTER key to confirm Wait for the measured value to stabilise the display shows END Once calibration is complet...

Страница 36: ...f the temperature reading displayed differs from that of an accurate thermometer 10 1 1 Press and hold the MODE key for 3 seconds to enter temperature calibration mode the display shows current temper...

Страница 37: ...minutes Clogged reference junction Heat a diluted KCl solution to 60 o C to 80o C Place the tip of the electrode into the heated solution for about 10 minutes Allow the electrode to cool in some room...

Страница 38: ...ectrode storage solution CLEANING THE ELECTRODE Contamination of the sensor often results in slow response and inaccurate readings If necessary clean the element by one of the following procedures Ino...

Страница 39: ...emoved very carefully with a cotton bud soaked in solvent taking care not to touch the metal parts of the inner cell 11 4 DO Probe Care and Maintenance Always keep probe membrane moist If dissolved ox...

Страница 40: ...whether clogged dirty or broken Incorrect calibration solutions Using the fresh calibration solutions for calibration Setting value does not match calibration solution Reset the calibration value pH...

Страница 41: ...key to set the temperature at 25o C 3 Dip the conductivity electrode and temperature probe into sample solution A 4 Record the temperature value TA and conductivity value CTA 5 Repeat the steps above...

Страница 42: ...TDS value of a precisely weighed quantity of anhydrous NaCl or KCl dissolved in high purity water Actual Conductivity the meter measured conductivity value For example Dissolve 64 grams of potassium c...

Страница 43: ...60 100 100 1 750 200 98 8 741 300 97 6 732 400 96 4 723 500 95 2 714 600 94 0 705 700 92 8 696 800 91 7 688 900 90 5 679 1000 89 4 671 1100 88 3 662 1200 87 2 654 1300 86 1 646 1400 85 0 638 1500 84 0...

Страница 44: ...red by the Trace2o Ltd standard warranty terms and conditions available via email or via download from www trace2o com In the event that any technical assistance is required Trace2o Customer service d...

Страница 45: ...45 NOTES...

Страница 46: ...46 Trace2o Limited The Technology Centre Wagtech Court Station Road Thatcham Berkshire RG19 4HZ Tel 44 0 1635 566772 Fax 44 0 1635 873509 sales Trace2o com www Trace2o com...

Страница 47: ...ring rod Ammonia Nos 1 2 tablets Safety Consult the safety data sheet for all of the reagents before use Even if you have used HydroTest reagents before the formulation may have changed Getting starte...

Страница 48: ...ia No 2 tablet straight from the foil to the water sample Crush the tablet using a clean stirring rod until no large pieces are visible Close the vial tightly with the cap and swirl several times unti...

Страница 49: ...the correct sequence Ammonia No 1 tablet will only dissolve fully after Ammonia No 2 tablet is added The tablets should be added direct from the foil avoiding contact with hands or surfaces The temper...

Страница 50: ...Trace2o Ltd Rev1 0...

Страница 51: ...ult the safety data sheet for all of the reagents before use Even if you have used HydroTest reagents before the formulation may have changed Getting started Switch the unit on using the power key Sel...

Страница 52: ...pieces are visible Close the vial tightly with the cap and swirl several times until the tablet is dissolved Analysis Free Chlorine Place the vial in the sample chamber making sure that the marks on...

Страница 53: ...Determination Place the vial in the sample chamber making sure that the marks on the instrument and vial are aligned Press the Test key Remove the vial from the sample chamber Add one DPD No 3 tablet...

Страница 54: ...chlorite solution for 1 hour and rinse with deionised water The tablets should be added direct from the foil avoiding contact with hands or surfaces The sample should have a pH value of between 6 2 an...

Страница 55: ...itrate Test tablet 10ml vial Stirring rod Nitrite LR tablet Safety Consult the safety data sheet for all of the reagents before use Even if you have used HydroTest reagents before the formulation may...

Страница 56: ...low contents to settle then gently invert three to four times Allow to stand for a further 2 minutes then open and wipe carefully around the rim to remove solid particles Decant 10ml of the treated so...

Страница 57: ...ct with hands or surfaces Nitrite present in the sample will also react leading to a higher result To correct carry out a nitrite test P270 on the same sample and subtract the result Nitrate concentra...

Страница 58: ...Trace2o Ltd Rev1 0...

Страница 59: ...Stirring rod Nitrite LR tablet Safety Consult the safety data sheet for all of the reagents before use Even if you have used HydroTest reagents before the formulation may have changed Getting started...

Страница 60: ...ater sample Crush the tablet using a clean stirring rod until no large pieces are visible Close the vial tightly with the cap and swirl several times until the tablet is dissolved Analysis Place the v...

Страница 61: ...free from fingerprints Always handle vials by the lid where possible The tablets should be added direct from the foil avoiding contact with hands or surfaces Interferences may occur from presence of t...

Страница 62: ...Trace2o Ltd Rev1 0...

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