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SD-OCT Base Unit

Chapter 6: Sample Applications

Page 54

MTN020880-D02

Figure 66: Images from Fluorophore Solution Dripped on Plastic Card Between Two Strips of Tape shows the
acquired images as seen in the ThorImage

OCT Software, when using the color overlay function for the data

from the analog input. To get a sufficient amount of signal, we used a custom OCT scanner with a broadband
reflecting silver mirror but without a reference arm. Instead, a coupler and a

separate

reference arm were added

to the scheme in Figure 64. Please contact

[email protected]

for details of this setup.

Scratch Bubbles Fluorophore Accumulation at the Rim

Raw OCT signal

Raw OCT signal with color-coded
overlay from analog input

Figure 66: Images from Fluorophore Solution Dripped on Plastic Card Between Two Strips of Tape

Содержание GAN1x1 Series

Страница 1: ...SD OCT Base Unit Spectral Domain OCT System Base Units Telesto and Ganymede Series User Manual...

Страница 2: ...Original User Manual not translated...

Страница 3: ...System Technology 19 Polarization Sensitive Optical Coherence Tomography 20 Nomenclature in OCT Imaging 21 OCT Base Unit Components 23 Base Unit 23 PC with Graphical User Interface 23 SDK 23 Imaging S...

Страница 4: ...aging Systems 59 Declaration of Conformity GAN3x1 Series Base Units 60 Declaration of Conformity GAN3x1C1 Imaging Systems 61 Declaration of Conformity GAN6x1 Series Base Units 62 Declaration of Confor...

Страница 5: ...re incorporated in the Telesto Series ATTENTION Check the supply voltage of the system before plugging in the computer Make sure the included power cords for the base unit computer and monitor are con...

Страница 6: ...d within the range of three meters of this unit since the electromagnetic field intensity may exceed the maximum allowed disturbance values according to IEC 61000 6 1 2005 The safety of any system inc...

Страница 7: ...perature Handle all connectors both electrical and optical with care Do not use unnecessary force as this may damage the connectors Handle the optical fiber with care Mechanical stress can decrease pe...

Страница 8: ...n be cleaned using the FBC250 2 5 mm Fiber Bulkhead and Connector Cleaner This fiber cleaner features an extended mode which is useful for panels with multiple bulkhead connectors or other tight space...

Страница 9: ...ket C Interlock Connector Shipped with Preinstalled Plug D Connection Port for OCTH Imaging Scanner LEMO 14 Pin E Connection Port for OCTG and OCTP Galvo Driven Imaging Scanners LEMO 19 Pin F FC APC F...

Страница 10: ...supply voltages Electrical Scanner Connection Interfaces Galvo Scanner Lemo ECG 2B 319 CLL OCTH Scanner Lemo ECA 1B 314 CLL Table 1 Electrical Scanner Connection Interface Please contact Thorlabs OCT...

Страница 11: ...mperature before attempting to operate Operate this system on a flat dry and stable surface only 1 Install the PC monitor mouse and keyboard according to the documentation provided by the PC manufactu...

Страница 12: ...come with different CameraLink configurations see J in Figure 3 The markings on the CameraLink cable s must match those on the frame grabbers and base unit a For the Telesto PS Series base units the t...

Страница 13: ...fixed to the scanner unit Align the red dot of the plug to the alignment mark of the electric connection port of the scanner shown here OCTG Figure 6 Plugging the Electric Connector into the Scanner F...

Страница 14: ...he fiber and clean it according to chapter 1 2 2 Figure 8 Connecting the Fiber to the Imaging Scanner A Slide the fiber tip into the center bore of the fiber connection B C The fiber needs to be orien...

Страница 15: ...Installing the Electric Connection Cable at the Base Unit Push the connector into the receptacle until a click sound is heard 10 Connect the fiber to the base unit Not required in Telesto PS series b...

Страница 16: ...he fiber connection B C The fiber needs to be oriented in rotation so that the alignment key slides into the mating part of the probe connector D Secure the fiber connection by turning the lock cap cl...

Страница 17: ...nnector This allows the trigger signal to be inserted into the existing connection as shown in Figure 12 This option is not available in Telesto PS Series base units ATTENTION To make use of the exter...

Страница 18: ...gger pulse will occur after every finished B scan of the continuous OCT imaging When using the trigger I O port configured as an input a trigger pulse initiates a single B scan ATTENTION To ensure cor...

Страница 19: ...SD OCT Base Unit Chapter 2 Setup Rev C June 25 2021 Page 15 Figure 13 SMA Cable Connected to Trigger I O port...

Страница 20: ...ng process SMA connectors Analog IN1 Analog IN2 on the back panel of the OCT base unit The sampled data can be saved to a file and visualized using the ThorImage OCT software application Please refer...

Страница 21: ...OCT systems and SS OCT for the time encoded systems In both types of systems a fiber coupler splits light into the sample and reference arms of an interferometer setup Back reflected light attributed...

Страница 22: ...the imaging depth will be doubled The signal width has two limits One limit for the signal width is given by the spectral distribution of the light source For a fully used Gaussian shaped light sourc...

Страница 23: ...where the unique phase delay for each wavelength is detected To gain access to this back scattered light it is necessary to insert a splitting device into the optical path In Thorlabs SD OCT systems t...

Страница 24: ...ates of both beams To achieve a good sensitivity for both polarization axes the polarization state of the reflected beam needs to be rotated by 45 when interfering with the sample beam To implement th...

Страница 25: ...the 2D Polarization Sensitive Mode chapter of the ThorImage OCT Software Manual Nomenclature in OCT Imaging Figure 19 A Scan Data Set As described before the SD OCT engine creates a depth profile fro...

Страница 26: ...t display modalities Please refer to the ThorImage OCT Software Manual for all features available Figure 21 Rendered Volumetric Data Set Cropping a 3D image to a plane with both scan directions as axe...

Страница 27: ...e unit include a high speed linear imaging sensor analog and digital timing circuitry analog controls for the probe and data acquisition hardware The central wavelength of the system depends on the SL...

Страница 28: ...B Scans or complete volumetric measurements Processing of acquired OCT data ranges from simple A Scan processing to advanced routines for Doppler speed measurements Some exemplary features enabled by...

Страница 29: ...associated with chromatic or polarization mode dispersion that are introduced by differences between individual fibers in the sample and reference arms Various lens kits are available for these scanne...

Страница 30: ...SD OCT Base Unit Chapter 3 Description Page 26 MTN020880 D02 Figure 26 OCTP PS User Customizable PS OCT Scanner Figure 27 OCTH Handheld Scanner...

Страница 31: ...021 Page 27 OCT STAND Accessory The Thorlabs OCTG and OCTP scanners can be mounted to an OCT STAND The rotation translation stage OCT XYR1 is available as a common accessory to the OCT STAND Figure 28...

Страница 32: ...T Base Unit Chapter 3 Description Page 28 MTN020880 D02 Figure 30 OCT XYR1 Sample Translation Rotation Stage For further details on the OCT STAND and the OCT XYR1 stage please refer to the Thorlabs we...

Страница 33: ...ed with respect to temperature and the related performance parameters The warm up takes approximately 10 seconds to complete Figure 31 Indicators and Buttons on the Base Unit Front Panel Note If any o...

Страница 34: ...SD OCT Base Unit Chapter 4 System Operation Page 30 MTN020880 D02 Figure 32 Basic Focus Adjustment Left Bad Focus Right Good Focus off focus in focus...

Страница 35: ...s at its optimum when the bar is green Adjusting the Reference Length The following steps will guide you to a basic adjustment of the reference length Start B scan acquisition When significantly misad...

Страница 36: ...l move the image up and down until you achieve the desired location Figure 35 B Scan of a Fingertip Out of Focus and In Focus In Figure 35 two images of a fingertip are shown the left is out of focus...

Страница 37: ...a more in depth analysis of the sample but also restricting the total axial depth that can be investigated For samples with a deep region of interest it is more helpful to choose an objective with a...

Страница 38: ...an Figure 37A After finding the focus autocorrelation terms will appear in the upper part of the image even without any reflecting surface in the field of view of the B scan When these artifact signal...

Страница 39: ...n of interest in the top half of the window e g because there is additional material on top of the region of interest that would flip back into the image and overlay with the region of interest In tho...

Страница 40: ...that provide a glass plate at a fixed distance to reduce scattering effects and to keep the sample in focus please contact Thorlabs for more information Figure 39 Plastic with Matte Surface Partially...

Страница 41: ...nce This technology is ideal for in line industrial imaging applications ranging from laminated packaging films to 3D visualization of mechanical parts Skin Imaging SD OCT provides real time high reso...

Страница 42: ...SD OCT Base Unit Chapter 4 System Operation Page 38 MTN020880 D02 Figure 42 B Scan of Skin and Nail Fold Top Intensity Based OCT image Bottom Optic Axis Polarization Sensitive OCT image...

Страница 43: ...gical material science applications SD OCT systems are ideal for monitoring surface topography and layered structures Figure 43 B Scan of a Semi Transparent Molded Plastic Cap Figure 44 B Scan of a La...

Страница 44: ...System Operation Page 40 MTN020880 D02 Biological Imaging Figure 46 B Scan of a Section of a Grape Figure 47 B Scan of carious tooth Top Intensity Based OCT image Bottom Degree of Polarization Unifor...

Страница 45: ...optical signal is interpreted as partially rectangular Consequently additional harmonic frequencies of the root signal appear Figure 48 High Surface Reflection Causing Saturation and Nonlinear Respons...

Страница 46: ...In the OCT software a colored bar indicates if the reference intensity is set properly Please refer to the OCTG standard scanner OCTP customizable scanner or the OCTH handheld scanner manual for infor...

Страница 47: ...etween interference created in respect to the reference arm and interference created within the sample Figure 51 shows the B scan of a sample laminated foils Figure 51 Autocorrelation from a Surface R...

Страница 48: ...per in the image than is physically true Figure 53 Schematic of a Setup to Show the Influence of Multiple Scattering Multiple scattering is intense in paper For illustration of this artifact a setup a...

Страница 49: ...A scan rate of f 1 25 kHz at 930 nm the maximum detectable speed v in the direction of the A scan axis is 290 m s When the direction of the movement occurs at an angle with respect to the A scan axis...

Страница 50: ...ared to the reference arm length When adjusting the reference length to be too long the image appears flipped Figure 56 Right Orientation Reference Length Adjustment for Imaging of an IR Viewing Card...

Страница 51: ...on of depth information this information can only be gleaned from photons exiting and reentering through the sampling aperture Reflections strong scattering and absorption lead to shadows in the depth...

Страница 52: ...tance from the beam splitter to the scattering or reflecting object These path lengths are optical path lengths calculated from the physical path length multiplied by the group refractive index See th...

Страница 53: ...on or envelope of the wave propagates through space This velocity usually is different from the speed of the phases of the single wavelengths This velocity is calculated by using the group refractive...

Страница 54: ...rement Depth With Refractive Media In the image the incoming beam from above is scanned over a structure made of two different materials named one d1 ng1 and two d2 ng2 stacked on a flat surface red l...

Страница 55: ...es of the materials assuming the underlying surface to be flat and horizontal in the image In the OCT image on the right the physical thicknesses i d optical path lengths i gid n as well as the result...

Страница 56: ...th and the displayed OCT image makes it difficult to perform an inverse ray tracing because all the diffractive interfaces need to be determined in 3D Even this determination needs to be undertaken st...

Страница 57: ...reflected excitation light and pass the fluorescence only We employed this idea to image a solution of fluorophore ATTO 647N with a Telesto OCT system the Ganymede will require different optical comp...

Страница 58: ...To get a sufficient amount of signal we used a custom OCT scanner with a broadband reflecting silver mirror but without a reference arm Instead a coupler and a separate reference arm were added to th...

Страница 59: ...Crashed Restart PC Poor Connection of USB Cable Check USB Connection Data Acquisition Cable Not Connected Connect Data Acquisition Cable Optical Path Length Not Matched Adjust Optical Path Length Bea...

Страница 60: ...Not Scanning Check with IR Card in Focus While Running a Slow 2D Scan Scanner Cable Connection Faulty Switch the System Off and Reconnect the Electrical Scanner Cable Between Base Unit and Scanner Sca...

Страница 61: ...replacement fuse s for the base unit Use only IS 1 A 250 VAC Type T 5x20 mm style fuses IEC 60127 2 III low breaking capacity slow blow Slide the fuse cover closed Figure 67 Fuse Cover on Base Unit R...

Страница 62: ...SD OCT Base Unit Chapter 8 Certifications and Compliance Page 58 MTN020880 D02 Chapter 8 Certifications and Compliance Declaration of Conformity GAN1x1 Series Base Units...

Страница 63: ...SD OCT Base Unit Chapter 8 Certifications and Compliance Rev C June 25 2021 Page 59 Declaration of Conformity GAN1x1C1 Imaging Systems...

Страница 64: ...SD OCT Base Unit Chapter 8 Certifications and Compliance Page 60 MTN020880 D02 Declaration of Conformity GAN3x1 Series Base Units...

Страница 65: ...SD OCT Base Unit Chapter 8 Certifications and Compliance Rev C June 25 2021 Page 61 Declaration of Conformity GAN3x1C1 Imaging Systems...

Страница 66: ...SD OCT Base Unit Chapter 8 Certifications and Compliance Page 62 MTN020880 D02 Declaration of Conformity GAN6x1 Series Base Units...

Страница 67: ...SD OCT Base Unit Chapter 8 Certifications and Compliance Rev C June 25 2021 Page 63 Declaration of Conformity GAN6x1C1 Imaging Systems...

Страница 68: ...SD OCT Base Unit Chapter 8 Certifications and Compliance Page 64 MTN020880 D02 Declaration of Conformity TEL2x1 PS Series Base Units...

Страница 69: ...SD OCT Base Unit Chapter 8 Certifications and Compliance Rev C June 25 2021 Page 65 Declaration of Conformity TEL2x1 PS C1 Imaging Systems...

Страница 70: ...SD OCT Base Unit Chapter 8 Certifications and Compliance Page 66 MTN020880 D02 Declaration of Conformity TEL3x1 Series Base Units...

Страница 71: ...SD OCT Base Unit Chapter 8 Certifications and Compliance Rev C June 25 2021 Page 67 Declaration of Conformity TEL3x1C1 Imaging Systems...

Страница 72: ...the specifications Non Warranty Repairs Products returned for repair that are not covered under warranty will incur a standard repair charge in addition to all shipping expenses This repair charge wi...

Страница 73: ...I O Connector Typical Output Signal Characteristics SMA Connector Type LVTTL Trigger Signal VL 0 2 V VH 2 5 V Pulse Length 200 ns 5 Rise Fall Time 25 ns 35 ns 5 50 Termination Required Analog IN1 Anal...

Страница 74: ...easured using a scanner with a common reference sample path and 50 path split Optical Performance Specifications Ganymede Series Base Unit Base Unit GAN111 GAN311 GAN321 GAN611 GAN621 Central Waveleng...

Страница 75: ...SD OCT Base Unit Chapter 11 Mechanical Drawings Rev C June 25 2021 Page 71 Chapter 11 Mechanical Drawings Figure 68 Base Unit Dimensions...

Страница 76: ...abs products such as Pure OEM products that means assemblies to be built into a unit by the user e g OEM laser driver cards Components Mechanics and optics Left over parts of units disassembled by the...

Страница 77: ...pport Contact If you have a technical question or issue on Thorlabs OCT products please refer directly to the OCT Support team located in Luebeck Germany OCT Support Thorlabs GmbH Maria Goeppert Stra...

Страница 78: ...Canada and South America Thorlabs Inc sales thorlabs com techsupport thorlabs com UK and Ireland Thorlabs Ltd sales uk thorlabs com techsupport uk thorlabs com Europe Thorlabs GmbH europe thorlabs co...

Страница 79: ......

Страница 80: ...www thorlabs com...

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