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iBright

™

Imaging Systems User Guide

Содержание Invitrogen iBright CL1000

Страница 1: ...n diagnostic procedures iBright Imaging Systems USER GUIDE For use with the iBright CL1000 Imaging System and the iBright FL1000 Imaging System Catalog Numbers A32749 CL1000 and A32752 FL1000 Publicat...

Страница 2: ...THIS DOCUMENT INCLUDING YOUR USE OF IT Revision history Pub No MAN0015702 Revision Date Description A 0 May 2017 New document Describes installation operation and maintenance of the iBright Imaging S...

Страница 3: ...the iBright imager 11 Choose imaging mode and start image capture 11 Chemi Blots 12 Capture an image of the sample 12 Fluorescent Blots FL1000 model only 13 Image using Fluorescent Blots FL1000 model...

Страница 4: ...ment 30 Prepare for shipping 30 APPENDIX A Instrument specifications 31 Instrument dimensions and specifications 31 Electrical requirements 31 Environmental requirements 32 Network requirements 33 Ins...

Страница 5: ...gnetic compatibility EMC standards 41 Safety compliance 41 EMC 41 Environmental design 42 Chemical safety 43 Biological hazard safety 44 Documentation and support 45 Related documentation 45 Customer...

Страница 6: ...id stains and visible protein gels The iBright FL1000 Imaging System adds the capability of imaging fluorescent Western blots using multiple dyes The iBright imagers use a simple design and intuitive...

Страница 7: ...ample placement Contents Table 1 Included imager system parts Component Quantity Power cord region specific 1 Quick reference card 1 White transilluminator screen 1 Reference target 1 Sample stage tur...

Страница 8: ...rofile to your Thermo Fisher Cloud account on page 8 Link a new profile to your Thermo Fisher Cloud account From your new My Profile screen 1 Touch Cloud 2 Enter your Thermo Fisher Cloud account usern...

Страница 9: ...le Note For those not logged on as a user guest acquired images are saved to a guest gallery Images cannot be transferred between user and guest galleries 3 Enter a Screen name 4 Enter and confirm a f...

Страница 10: ...transferred between user and guest galleries 2 From the Welcome screen choose the appropriate Mode from the drop down menu touch Open Drawer and place sample in the center of the turntable glass 3 Tou...

Страница 11: ...n drawer 3 Place sample in the center of the viewing area 4 Touch to close drawer Instrument automatically aligns zooms focuses and acquires a live view sample image To image in Chemi Blots mode proce...

Страница 12: ...then renders a preview image and recommends an exposure time This is not a real image 2 Optional Adjust the exposure time using the following methods Touch or Finger swipe in the segmented dial Touch...

Страница 13: ...time or touch Custom to manually input a time Note To adjust or set the exposure time for a specific dye channel see Edit dye channel exposure time on page 14 5 Touch Enter The image preview will upda...

Страница 14: ...To adjust or set a manual exposure time select a method Touch or within dial Finger swipe in the segmented dial Touch the dial center box to select a preset exposure time or touch Custom to input a u...

Страница 15: ...ch or within dial Finger swipe in the segmented dial Touch the dial center box to select a preset exposure time or touch Custom to manually input a time 3 Touch Enter The image preview will update in...

Страница 16: ...series of short exposures and then renders a preview image and a recommended exposure time This is not a real image 2 To adjust or set a manual exposure time select a method Touch or within dial Fing...

Страница 17: ...period Signal accumulation Series of exposures where user defines the first capture time interval the last capture cumulative capture time and the total number of captures Signal accumulation Note Im...

Страница 18: ...Manual exposure Manually select an exposure time for sample Smart exposure Predicts optimal exposure for minimizing pixel saturation and maximizing dynamic range for a specific sample Renders a previe...

Страница 19: ...splayed as different false colors overlaid on a black background Individual channels can be toggled on and off in the composite image by touching Edit channels and on the left of the appropriate dye 1...

Страница 20: ...the Welcome screen or mode drop down menu 1 Touch Gallery or 2 Toggle between Thumbnail view and List view to see stored images as thumbnails or a table Click the column headers to sort the images 3 T...

Страница 21: ...information may be required to map a network drive Drive location Enter the IP address server name and file pathway use forward slashes Example 192 168 1 100 shared R D Data John_Smith 2016 Additional...

Страница 22: ...rom the Export images screen 1 Touch Choose File Type 2 Touch Publication or Analysis to select the intended use 3 Touch a file type File type Description Publication Exports 24 bit color adjusted ima...

Страница 23: ...isting analysis frames 5 Touch Apply to accept any changes 6 Optional for fluorescent images Touch the dye drop down menu to select a particular dye or the membrane view 7 Optional Touch More options...

Страница 24: ...sus the molecular weight and relative mobility of known standards On the Welcome screen or capture screen Mode drop down menu 1 Touch Gallery or Select one or multiple images 2 Touch Actions 3 If more...

Страница 25: ...top of the table touch to change the table output by Output Units Description Volume Intensity Sum of pixel intensities contained in an identified band Area Pixels Total number of pixels contained in...

Страница 26: ...Name Customize the instrument name Language Select a display language subject to availability Reset User Tips Reset user tips for the logged in guest Reset Factory Defaults Resets instrument to facto...

Страница 27: ...turntable for shipping Note Instrument can only be powered off once turntable is removed Once powered off then powered on you must go through the complete new instrument set up again Manage accounts a...

Страница 28: ...r is not blocked The drawer will open as part of the testing Clean the instrument Repeated instrument use can cause spots and smudges on the sample stage which can then decrease image quality Clean th...

Страница 29: ...older and inspect it for damage Carbon typically coats the inside of failed fuses 4 Replace each failed fuse with a 1 6A Time Lag T 250VAC 5 20 mm Fuse Note The voltage and amperage ratings are on the...

Страница 30: ...y Ensure that the surface on which you place the instrument can support at least 50 kg 110 lbs Ensure that the path to transport the instrument is clear of obstructions At least two people are needed...

Страница 31: ...low adequate space between the wall and the equipment so the power cord can be disconnected in case of emergency Electric receptacle with grounding capability Maximum power dissipation 960 W not inclu...

Страница 32: ...al output based on the actual current draw of the instrument is expected to be approximately 960 W 3275 Btu h Vibration Ensure that the instrument is not adjacent to strong vibration sources such as a...

Страница 33: ...is to install the instrument If the instrument will be connected to a LAN an active tested network jack must be in place before the scheduled installation date A representative from your information...

Страница 34: ...computer connections iBright Imager front view 1 2 3 1 Touchscreen 2 USB port 3 Sample stage turntable Appendix A Instrument specifications Instrument and computer connections A 34 iBright Imaging Sys...

Страница 35: ...Ethernet port RJ45 port for 10 100 Mbps ethernet communication with the instrument 3 Fuse cover 4 Power switch 5 Power port 100 240 VAC Appendix A Instrument specifications Instrument and computer con...

Страница 36: ...ent Symbols may be found on the instrument to warn against potential hazards or convey important safety information In this document the hazard symbol is used along with one of the following user atte...

Страница 37: ...ri s lectif Se conformer aux ordonnances locales sur les d chets municipaux pour les dispositions d limination et communiquer avec le service la client le pour des renseignements sur les options d lim...

Страница 38: ...f you remove the protective instrument panels or disable interlock devices you may be exposed to serious hazards including but not limited to severe electrical shock laser exposure crushing or chemica...

Страница 39: ...y that the fuses are properly installed and the fuse voltage matches the supply voltage Replace fuses only with the type and rating specified for the unit Improper fuses can damage the instrument wiri...

Страница 40: ...ty or sending the instrument for repair maintenance trade in disposal or termination of a loan decontamination forms may be requested from customer service Before using any cleaning or decontamination...

Страница 41: ...nt for measurement control and laboratory use Part 2 081 Particular requirements for automatic and semi automatic laboratory equipment for analysis and other purposes IEC 62471 1 2007 Photobiological...

Страница 42: ...se the user will be required to correct the interference at his own expense Reference Description EU Directive 2012 19 EU European Union WEEE Directive Waste electrical and electronic equipment EU Dir...

Страница 43: ...ersonal protective equipment when handling chemicals for example safety glasses gloves or protective clothing Minimize the inhalation of chemicals Do not leave chemical containers open Use only with a...

Страница 44: ...nd company institution requirements before working with potentially biohazardous materials Follow all applicable local state provincial and or national regulations The following references provide gen...

Страница 45: ...ems touchscreen and provides procedures for configuring the instrument and performing imaging Customer and technical support Visit thermofisher com support for the latest in services and support inclu...

Страница 46: ...hnologies General Terms and Conditions of Sale found on Life Technologies website at www thermofisher com us en home global terms and conditions html If you have any questions please contact Life Tech...

Страница 47: ......

Страница 48: ...thermofisher com support thermofisher com askaquestion thermofisher com 12 May 2017...

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