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Model 3010 Incubator ________________________________________________________Installation and Start-Up
1 - 6
i. Filling the Water Jacket
Turn the power switch on. ADD WATER will appear in the
message center. Press the Silence key to silence the alarm.
Note:
The fill port has a plug that must be removed before fill-
ing and replaced after filling is complete.
Chlorine is detrimental to stainless steel. Using
chlorinated tap water will void the water-jacket
warranty!
Note:
High purity water (1M to 18M ohm/cm resistivity) is a
very aggressive solvent and is considered slightly acidic. Ideal
pH for the water in the jacket is 7. Sodium hydroxide may be
used to change the pH of high purity water. It requires approxi-
mately 8ml of 0.05 normal sodium hydroxide per gallon of high
purity water to raise the pH to 7. The water jacket holds appox-
imately 12 gallons. Sodium hydroxide and the rust inhibitor
may be used in the same water jacket.
Fill the water jacket with 11.7 gallons (43.5 liters) of dis-
tilled water with a resistance range of 50K to 1M Ohm/cm
(conductivity range of 20.0 to 1.0 uS/cm). Silicone tubing and a
funnel are included in the accessory bag shipped with the unit.
For ease of connecting/disconnecting, use the silicone tub-
ing provided to connect directly to the fill port. See Figure 1-1
and this detail. Do not install vinyl
tubing directly onto the fill port as
it may be difficult to remove. A
3/8” to 3/8” hose connector has
been provided to attach lengths of
vinyl tubing to the silicone fill port
tubing, to reach the distilled water
source.
When the jacket is full, the audible alarm will sound a con-
tinuous tone for 10 seconds and the alarm condition will be
cleared. Refer to Section 4.1, Table of Alarms.
Note:
Model 3010 Water Jacket Incubator is shipped from the
factory with a rust inhibitor added to the water inside the unit.
The rust inhibitor must be replenished every 2 years
. See
Section 5.9 to drain the water jacket and Section 5.12 for the
correct proportion of rust inhibitor to the water.
j. Filling the Humidity Pan
For best operation of the incubator, sterilized distilled,
demineralized or de-ionized water should be used in the humid-
ity pan. Water purity should be in the resistance range of 50K
Ohm/cm to 1M Ohm/cm, or a conductivity range of 20.0 uS/cm
to 1.0 uS/cm. Refer to ASTM Standard D5391-93 or D4195-88
for measuring water purity.
Distillation systems, as well as some types of reverse
osmosis water purity systems, can produce water in the quality
range specified. Chlorinated tap water is not to be used as chlo-
rine can deteriorate the stainless steel. Tap water may also have
a high mineral content, which would produce a build-up of
scale in the reservoir. High purity, ultra pure or milli-q water is
not recommended as it is an extremely aggressive solvent and
will deteriorate the stainless steel. High purity water has a
resistance of above 1M to 18M Ohm/cm. Even high purity
water can contain bacteria and organic contaminants. Water
should always be sterilized or treated with a decontaminant,
safe for use with stainless steel as well as safe for the product,
prior to being introduced into the humidity pan.
Distilled or de-ionized water used in the humidity
pan must be within a water quality resistance
range of 50K to 1M Ohm/cm to protect and pro-
long the life of the stainless steel. Use of water out-
side the specified range will decrease the operating
life of the unit and void the warranty.
Fill the humidity pan to within 1/2 inch of the top with
sterile, distilled water. Place the pan directly on the incubator
floor to ensure optimum humidity and temperature response.
For applications requiring higher humidity conditions, the
pan should be placed against the left side wall of the incubator.
The ductwork has been modified for this purpose. Also, the CO
2
sample port may be capped to assist in achieving greater RH. In
some ambients, this may cause condensation to form in the
chamber.
Check the level and change the water frequently to avoid
contamination. Do not allow the water level to fluctuate signifi-
cantly. “Dry-outs” will have an adverse effect on the humidity
level.
Detail 1
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