Thermo Scientific Invitrogen Qubit Flex Fluorometer, Руководство пользователя

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Qubit ™ Flex Fluorometer User Guide
97
Appendix A: Troubleshooting
Troubleshooting
Handling samples •
The calibration standards included in the Qubit ™ microRNA, Qubit ™ RNA HS,
and Qubit
™
RNA BR Assay Kits are high-quality RNA standards. The integrity
and concentration of these standards is critical to the optimal performance of
the Qubit
™ RNA assays. We highly recommend treating the rRNA standards
as you would any other precious RNA. Use appropriate RNase-free handling
techniques, including RNase-free gloves, pipette tips, and tubes. Keep the tube
lids closed whenever possible; do not press the pipet to the inside wall of the
tube when withdrawing a sample. Return the RNA standards to –80°C as soon
as possible after use.
•
Ensure that the assay tubes are at room temperature at the time the reading is
taken. Do not hold assay tubes in your hand and do not leave assay tubes in
the Qubit
™ Flex Fluorometer for longer than it takes to read the fluorescence.
See “Assay temperature”, page 100.
•
Be careful not to spill sample into the sample chamber. Promptly wipe any
spills.
•
The Qubit ™ assays are very sensitive and even small amounts of material
from a previous sample may result in errors. Use a clean Qubit ™ Flex Tube
Strip for each reading.
•
The tube must be clean and dry on the outside when taking readings.
Moisture and condensation on the tube surface can lead to reading errors.
•
Minute bubbles in samples will cause errors in readings. Be sure not to
introduce bubbles into samples. Slight tapping on the tube wall or brief
centrifugation will often help dissipate bubbles.
High reading •
The sample is out of range. Use a
sample that is less concentrated or add a
smaller volume of sample into the assay
to further dilute the sample.
•
For Qubit ™ quantification assays, view
the Fluorescence vs. Concentration
graph in the Results screen to confirm
that the values for the samples fall
between the values of the standards
(page 43).
•
Ensure that the lid is closed while
reading standards and samples.
•
Prepare samples and standards
according to the instructions in the
Qubit
™ assay kit you are using.
•
Ensure that the assay is performed
entirely at room temperature.