Qubit
™
Flex Fluorometer User Guide
37
3.
Press
Next
to go to the Sample volume screen.
4.
In the
Sample volume
screen, enter the
sample volume
added to the assay
tube (between 1 and 20 µL).
You can enter the volume directly in the sample volume text box, use the
+
and
–
buttons, or adjust the sample volume wheel.
When you enter the sample volume, the assay range information on the
screen automatically changes to reflect the new core and extended accuracy
ranges based on the sample volume.
dsDNA HS Assay range
for 10 µL sample volume
dsDNA HS Assay range
for 20 µL sample volume
Note:
The sample volume used (1–20 µL) changes the assay accuracy
range. For highest accuracy, use the maximum sample volume that
would keep the concentration measurements within the core range.
If the sample concentration is outside of what the assay can accurately
quantify, a different sample volume or assay may be required.
