Qubit
™
Flex Fluorometer User Guide
23
Guidelines for using the Qubit
™
Flex Fluorometer
Recommendations
To obtain the best results, follow the recommendations below. For more
information, see “Critical Qubit
Assay considerations”, page 99.
•
Do
not
operate the instrument in direct sunlight.
•
Wear gloves during sample handling.
•
Use the instrument at room temperature only (22–28ºC).
•
Bring all kit reagents to room temperature and insert all assay tubes into the
instrument only for as much time as it takes for the instrument to measure the
fluorescence.
•
Do not hold the assay tubes in your hand before performing a measurement.
•
Make sure that you have calibrated the Qubit
™
Flex Fluorometer using the
appropriate standards.
•
The assay volume must be 200 µL for an accurate read.
•
Take care not to create air bubbles when mixing the sample or standard with
the working solution.
•
Incubate the tubes for the Qubit
™
DNA and RNA assays for 2 minutes after
mixing the sample or standard with the working solution.
•
Incubate the tubes for the Qubit
™
protein assays for 15 minutes after mixing
the sample or standard with the working solution.
•
If you are performing multiple readings of a single tube, remove the tube
from the instrument and let it equilibrate to room temperature for 30 seconds
before taking another reading.
Note:
Multiple readings of RNA samples are not recommended.
•
for additional application notes, technical notes,
citations, software updates, and a list of validated Qubit
™
assays that have
been tested using the Qubit
™
Flex Fluorometer.
Assay tubes for
the Qubit
™
Flex
Fluorometer
Only thin-wall, clear 0.2-mL PCR tube strips are appropriate for use in the Qubit
™
Flex Fluorometer. For best results, we recommend using Qubit
™
Flex Tube Strips
(Cat. No. Q33252).
