Thermo Scientific Invitrogen AMQAX2000, Руководство пользователя

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6.
Press Count .
The instrument captures the image and displays the results (total concentration, percentage, and
concentration of live and dead cells). For more information, see “View results”
on page 24 .
Next steps
• To identify the objects (i.e., cells) counted as Live, press . To identify objects counted as Dead,
press . See “Identify cells counted in cell count and cell viability assays” on page 25 .
Note: The Live and Dead buttons are enabled by default after the image has been counted.
• To see the distribution of live and dead cells in a graphical format, press
Histogram . See
“Histogram count results” on page 26 .
• To gate the results by object size, brightness, or circularity, press Gating to go to the Gating
screen. See “Gate count results” on page 26 .
Note: You can save the changes you make to the size, brightness, or circularity parameters in the
Gating screen to the current template or as a separate template. See “Save as new template” on
page 27 .
• To calculate the volume of cell sample and buffer needed to reach a desired concentration based
on the count results, press Calculators to open the Calculator applications. See Chapter 6,
“Calculators”.
• To permanently save the results, press
Save . See Chapter 7, “Save results”.
• To perform a new count, push the slide to eject, then insert a new sample slide.
Chapter 5
Cell count and cell viability assays
Count cells in brightfield
5
Countess ™ 3 Automated Cell Counter User Guide
23