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Thermo Scientific Invitrogen AMQAX2000, Руководство пользователя

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Calculators

Pre-Dilution Calculator

The 

Pre-Dilution Calculator

 is used to account for diluting a very concentrated sample before loading

it into a sample slide for counting.

For a brightfield count, this calculator also has an option to account for trypan blue use.

1.

On the 

Results

 screen, press 

Calculators

 then select 

Pre-Dilution Calculator

.

2.

Click in one of the text fields.

Note: 

For BF (brightfield) counts using trypan blue, you can select the checkbox at the bottom of

the screen to correct for a 1:1 trypan blue dilution.

6

28

Countess

™

 3 Automated Cell Counter User Guide

Содержание Invitrogen AMQAX2000

Страница 1: ...For Research Use Only Not for use in diagnostic procedures Countess 3 Automated Cell Counter USER GUIDE Catalog Number AMQAX2000 Publication Number MAN0019566 Revision A 0...

Страница 2: ...ITIVE MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT INCLUDING YOUR USE OF IT Revision history Pub No MAN0019566 Revision Date Description A 0 09 November 2020 New...

Страница 3: ...strument with a new Connect account 9 Sign in to the instrument with an existing Connect account 11 CHAPTER 3 Templates 12 Templates screen 12 Template functions 13 Count parameters 13 Load a template...

Страница 4: ...screen 26 Gate count results 27 Save as new template 27 Edit and save a new template 27 CHAPTER 6 Calculators 28 Pre Dilution Calculator 28 Cell Splitting Calculator 29 CHAPTER 7 Save results 31 Save...

Страница 5: ...l specifications 45 Physical characteristics 45 Technical specifications 45 Optics 45 Analysis slide 46 APPENDIX C Countess 3 Automated Cell Counter and accessories 47 Accessory products 47 APPENDIX D...

Страница 6: ...art optics and image analysis algorithms to analyze trypan blue stained samples in suspension The Countess 3 Automated Cell Counter offers an intuitive user interface with the option to save data and...

Страница 7: ...d Cell Counter It contains the buttons for all instrument functions and displays data from the cell count 2 Front USB port Allows you to transfer and save the cell count data and image to an external...

Страница 8: ...strument from the wall outlet Install the instrument 1 Unpack the instrument and place the instrument on a flat level dry surface 2 Remove the thin plastic protector film from the touch screen display...

Страница 9: ...ates Templates allows you to create customized count preferences i e gate counts based on cell size brightness and or circularity See Load a template on page 14 4 To change instrument settings press S...

Страница 10: ...ng code to enter online in your Thermo Fisher Connect account 4 A completion screen will show Press Done You then return to the Home screen and are logged into your account Once set up you will then o...

Страница 11: ...ccount 1 Press Sign in from the Home screen 2 Select your Username from the drop down box Enter your PIN 3 Press Sign in Chapter 2 Installation Sign in to the instrument with an existing Connect accou...

Страница 12: ...ameters are defined in the Edit template screen See Create a template on page 14 or Edit a template on page 14 The Default template contains default count settings and cannot be edited The count param...

Страница 13: ...ing the bottom gate up removes smaller objects moving it down includes smaller objects 2 Brightness Gating Moving the top gate down removes brighter objects moving it up includes brighter objects Movi...

Страница 14: ...ext box to open the keypad 3 Type in the desired template name To enter symbols press the symbol key To return to the alpha numeric keypad press ABC 4 Press Enter to save the name and return to the Ne...

Страница 15: ...plate and press Load to use that template to read the sample Import a template 1 Press Templates 2 Press Actions 3 Press Import 4 Press the import location Cloud or USB a For Cloud press Sign in to lo...

Страница 16: ...ss Actions 3 Press Export 4 Select one or more templates from the list 5 Press Next 6 Press the export location Cloud or USB a For Cloud press Sign in to log in to your Thermo Fisher Connect account I...

Страница 17: ...or USB insert your USB drive into the slot on the front of the instrument 7 Press Export 8 Once complete press Done Chapter 3 Templates Export a template 3 Countess 3 Automated Cell Counter User Guide...

Страница 18: ...ure that the counting area is covered with the cell suspension and count the cells immediately after staining per the assay protocol Do not press the optical surfaces of the chamber slides Hold the sl...

Страница 19: ...Cell count and cell viability assays 5 To remove the slide push the slide gently into the instrument until it clicks and the slide springs out Grasp the slide and pull it out the rest of the way Note...

Страница 20: ...ting Chamber Slide or the Countess Reusable Slide has a 10 L sample capacity Do not overfill the slide chambers 3 Load the reusable glass slide into the reusable slide holder 4 Insert the reusable sli...

Страница 21: ...cond sample is aligned with the sample viewing hole 8 After using the Countess Reusable Slide rinse the glass slide and cover slip with water and then clean with 70 ethanol Use Kimwipes laboratory tis...

Страница 22: ...ter settings as described in Load a template on page 14 3 Insert the sample slide into the slide port making sure that the sample side is inserted completely into the instrument You will hear a soft c...

Страница 23: ...gate the results by object size brightness or circularity press Gating to go to the Gating screen See Gate count results on page 26 Note You can save the changes you make to the size brightness or cir...

Страница 24: ...trypan blue and takes this dilution into account when calculating the total cell concentration The cell concentration displayed in the Results screen is the original cell concentration before dilution...

Страница 25: ...ailed image below shows both Live green and Dead red cells are selected 3 To unmark the cells press the appropriate colored button again Individual cells can be selected to view their specific size br...

Страница 26: ...n size only in the selected population 3 Optional Press Gating Using the Size Brightness and Circularity sliders adjust the count parameters As you adjust the count parameters the count results and th...

Страница 27: ...unt you may save your settings to a new template 1 Press Templates 2 To create a new template with the edited parameters press Create The new template screen opens and displays the edited count parame...

Страница 28: ...field count this calculator also has an option to account for trypan blue use 1 On the Results screen press Calculators then select Pre Dilution Calculator 2 Click in one of the text fields Note For B...

Страница 29: ...ll appear at the top of the Results screen Cell Splitting Calculator The Cell Splitting Calculator is used to determine the amounts of sample and buffer needed to reach a desired concentration 1 On th...

Страница 30: ...s and concentrations needed for your experiment Press Enter after filling in the text boxes 4 The calculation will automatically update below the text fields When completed press Done Chapter 6 Calcul...

Страница 31: ...format Note A dropdown list will provide options for how many counts to save You can choose from Current Capture Session Today Yesterday Past 7 days Past 30 days or All FCS Saves the data from the exp...

Страница 32: ...o the Save screen without saving the name press Close 5 Select the desired file type to save your experiment Result Images CSV Report Cell Data You can select an individual mode e g Result only or any...

Страница 33: ...days or All After you make your selection the instrument returns to the Save screen To return to the Save screen without changing the file format press Back 8 Press Save to save your experiment to the...

Страница 34: ...ration of the sample and the percentage and number for the total Live and Dead channels Below the results table the report contains the number of cells vs cell size graph Under the graph the report co...

Страница 35: ...ew one Software update Guidelines for software update Software updates can be done via your Connect account When connected to the internet the system will automatically check for and download software...

Страница 36: ...ve into one of the USB ports of the instrument 4 Press Settings on the Home page to open the Settings screen 5 Press Software Update 6 Select the update location Press Next If the Cloud option is disc...

Страница 37: ...he Edit Date keypad 6 Using the keypad enter the date into the Month Day and Year text boxes Press Enter 7 Press any Time text box Hours or Minutes to open the Edit Time keypad 8 Using the keypad ente...

Страница 38: ...r cloud region 1 Press Settings on the Home page 2 Press Instrument Settings 3 Press Cloud region Your current cloud region is displayed at the bottom of the screen 4 Select the desired cloud region f...

Страница 39: ...incompatible power adapter is not covered by warranty CAUTION Never disassemble or service the instrument yourself Do not remove any covers or parts that require the use of a tool to obtain access to...

Страница 40: ...Counter using a soft lint free cloth moistened with an LCD cleaning solution Do not apply excessive force during cleaning Wipe the touchscreen dry immediately after cleaning Ensure that the cleaning...

Страница 41: ...ch solution because it may leave a residue of bleach crystals on the instrument Ensure that water or other cleaning solutions do not enter the power button the power inlet the slide port or the USB po...

Страница 42: ...mal Gate out the debris using the size brightness and circularity sliders Autofocus does not focus properly Debris or other material interfered with the focus Make sure there are no bubbles or debris...

Страница 43: ...properly Make sure the USB drive is formatted to FAT32 If it is not reformat the USB drive to FAT32 before transferring the files onto the USB drive for software update Make sure the update file is at...

Страница 44: ...ach time a slide is inserted Depending on the concentration and uniformity of the cells this will cause some variability when performing replicate counts of the same slide although it should be less t...

Страница 45: ...or light industrial Pollution degree 2 Operating temperature 10 40 50 104 F Operating humidity 80 non condensing Technical specifications Processing time 15 seconds Sample concentration range 1 104 1...

Страница 46: ...sis slide Material Poly methyl methacrylate PMMA Dimensions 25 mm W 75 mm D 1 7 mm H Chamber volume 10 L Appendix B Technical specifications Analysis slide B 46 Countess 3 Automated Cell Counter User...

Страница 47: ...A48208 Accessory products The following products can be used with the Countess 3 3 FL Automated Cell Counters and are available separately from Thermo Fisher Scientific For more information visit www...

Страница 48: ...lue stain 0 4 2 1 mL T10282 1 Each box of Countess Cell Counting Chamber Slides contains 50 slides and 2 1 mL vials of trypan blue 0 4 sufficient for 100 counts Appendix C Countess 3 Automated Cell Co...

Страница 49: ...Blue Brightfield F Trypan blue corrected Sample with trypan blue correction applied G Pre Dilution corrected Sample with pre dilution correction applied H Total concentration Concentration of the enti...

Страница 50: ...tment slider value for minimum brightness AQ Dead brightness max Dead adjustment slider value for maximum brightness AR Dead circularity min Dead adjustment slider value for minimum circularity AS Dea...

Страница 51: ...tion provided in this document Before using an instrument or device read and understand the safety information provided in the user documentation provided by the manufacturer of the instrument or devi...

Страница 52: ...rs A primary waste container holds the immediate waste A secondary container contains spills or leaks from the primary container Both containers must be compatible with the waste material and meet fed...

Страница 53: ...les Individuals should be trained according to applicable regulatory and company institution requirements before working with potentially biohazardous materials Follow all applicable local state provi...

Страница 54: ...protocols Certificates of Analysis Safety Data Sheets SDSs also known as MSDSs Note For SDSs for reagents and chemicals from other manufacturers contact the manufacturer Limited product warranty Life...

Страница 55: ......

Страница 56: ...II Automated Cell Counter_UG_MAN0019566 v1 GUID E155E429 EBAF 4CA8 9CE4 7F018EEE3A3E 2020 09 08 22 16 53 en 21 11 12 359Z thermofisher com support thermofisher com askaquestion thermofisher com 9 Nove...

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