Thermo Scientific Invitrogen AMQAX2000, Руководство пользователя

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Gate count results
1.
On the Results screen, press Gating in the bottom right corner to open the Gating screen.
2.
(Optional) Press Histogram to view the distribution of cells (live and/or dead) based on size as
you gate the count results. See “Gate count results” on page 27 .
3.
Select the channel (Live or Dead ) you wish to gate.
4.
Select the count parameter (size, brightness, or circularity) to be changed.
5.
Using the start and end gate sliders, adjust the count parameters.
Note:
For a description of the count parameters and count parameter controls (i.e., parameter
sliders), see “Count parameters” on page 13 .
6.
When finished, press Apply to set the changes to the parameters and return to the Results screen.
7.
To save the parameter changes to the current template or to create a template with the new
parameters, see “Create a template”
on page 14 .
Save as new template
Edit and save a new template
If you have made changes to the count parameters after performing a count, you may save your
settings to a new template.
1.
Press Templates .
2.
To create a new template with the edited parameters, press Create . The new template screen
opens and displays the edited count parameters from the Gating screen.
3.
To update an existing template with the edited parameters, select a template from the list, then
press
Actions
4
Edit .
Note:
You cannot edit the Default template.
The Edit Template screen opens. To import the current settings from the Gating screen, press
Import settings .
4.
To change the name of the selected template, press the Template name text box and enter the
desired name using the alphanumeric keypad.
5.
(Optional) If desired, make additional changes to the template and the count parameters.
6.
Press Save to save the new template settings and return to the Templates screen. Press Load to
apply the template to your current and future counts OR press Cancel or Back to return to the
Templates screen, then Cancel or Back again to return to the Results screen without saving
the changes to the template.
Chapter 5
Cell count and cell viability assays
Save as new template
5
Countess ™ 3 Automated Cell Counter User Guide
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