4
2. Locate the outer support container in the area for
the cell culture run.
3. When monitoring the batch volume, the unit may be
placed on a weight scale if load cells are not part of
the system. Other methods may be used to measure
all incoming and outgoing liquids.
4. Level the platform by disabling the swivel casters on
the bottom of the outer support container. This is
accomplished by threading the leveling feet (at the
center of each caster) to the floor.
5. Verify the location of the pH/DO controllers and
ensure that the cable and tubing lengths are
sufficient.
6. Verify that the main power is off and the emergency
stop (E-Stop) is pulled out.
Note:
The E-Stop
disconnects all power to the system. An alarm
buzzer will sound when the E-Stop is activated.
7. Verify that the main motor power switch is in the
“off” position.
8. Connect all electrical plugs to facility power. Refer to
hardware/electrical labels and schematics to ensure
proper electrical voltage is connected to the S.U.B.
The main power switch can now be turned on.
9. Connect the inlet and outlet lines from the
temperature control unit (TCU) quick-connects to
the jacket (Figure 4). The inlet is typically on the right
side if you are facing the connectors; refer to the
permanent labels on the inlet and outlet lines.
Figure 4. Attaching water jacket port using a
tri-clamp.
Note:
Refer to the TCU manufacturer’s guidelines for
detailed TCU setup information.
10. Use the tri-clamp fitting to attach the water jacket inlet/
outlet ports to the bottom of the outer support container
(Figure 5).
Figure 5. Removing the tri-clamp and attaching the fitting.
Note:
Figure 5 shows the tri-clamp fitting and inlet/outlet
ports on a standard HyPerforma S.U.B. The location and
process is the same for DynaDrive S.U.B.s.
Loading the BPC
Use the following information to install and set up the BPC.
Checkpoints prior to BPC loading
9
A 500 L DynaDrive BPC is being used for the
500 L outer support container.
9
The outer support container is stationary, with the
casters locked in place.
9
Two operators are available for BPC loading.
9
The Thermo Scientific BPC Unpacking and Inspection
Guide (DOC0021) has been reviewed for information on
handling, transporting, and storing BPCs.
1. Open the door on the bioreactor outer support
container.
2. Use a safety cutter to slit the taped sides/ends of the
cardboard box in which the BPC is shipped (Figure 6).
The BPC will be secured to a cardboard insert inside the
box (Figure 7).