Thermo Scientific HM525 NX Operator Guide
60
Problem
Cause
Removal
Sticky sections
Specimen not cold enough
Select a lower temperature
Knife and/or anti-roll plate are not
yet cold enough – section melts
Allow knife and/or anti-roll plate to cool.
Specimen incorrectly clamped.
Blade clamping force can be increased by
tightening. Wait until the knife and/or the anti-
roll plate have sufficiently cooled.
Check specimen is securely mounted and
clamped in specimen head jaws. Check there is
no debris or ice on the back of the chuck or on
specimen head.
Sections do not stretch,
although the correct
temperature has been
selected and the anti-roll
plate has been adjusted
correctly
Knife and/or anti-roll plate are not
clean
Clean with a dry cloth or brush
Edge of anti-roll plate is damaged
Replace anti-roll plate
Blunt knife
Move / replace / sharpen the knife
Sections break, fissures in
the sections, sections are
not stretched properly
Specimen too cold
Select a higher temperature
Static charge/draught
Remove the cause
Specimen not cold enough
Select a lower temperature
Large specimen
Trim the specimen in a parallel way; select a
thicker section thickness
Anti-roll plate not adjusted
properly
Readjust the anti-roll plate
Anti-roll plate not aligned parallel
towards the knife edge
Align Anti-roll plate parallel to knife edge
Incorrect clearance angle
Readjust the clearance angle
Blunt knife
Move / replace / sharpen the knife
