Thermo Scientific HM525 NX Operator Guide
30
Setup
Before sectioning, the blade holder and the cryo chamber should be at a stable temperature close to the
desired cutting temperature. These temperatures should be selected to suit the consistency of the
specimen.
All tools which come into contact with the sections or which are used to manipulate the specimen must
also be cooled to prevent the tissue from sticking to them. Therefore all accessories should be stored on
the shelves or in the brush tray in the cryo chamber. The exceptions to this rule are the specimen chucks
which should be kept at room temperature.
The heated sliding window should be closed during periods of inactivity and while preparing specimens
to avoid warm air getting into the chamber. This will result in more favourable working conditions and a
lower frost build-up.
The surface of the blade holder base might be cold. Use gloves if necessary.
Note
The clearance angle can be read on the scale on the left side on the upper part of the blade holder.
Note
Usable cuts are only achieved at a clearance angle of 10° or more.
