EVOS
™
M5000 Imaging System User Guide
43
4.
Select
Z-Stack
to capture multiple images along the Z-axis based on your
specifications.
Unselect Z-Stack to capture images only at the focus position set for each
channel (either Auto Focus as part of the routine or initial focus positions).
5.
If you have the optional EVOS
™
Onstage Incubator installed
and want to use it during your time lapse routine, select
Use Incubator
.
This option is only available if you have installed and connected the EVOS
™
Onstage Incubator to your EVOS
™
M5000 instrument.
IMPORTANT!
Before using the EVOS
™
Onstage Incubator in your time lapse
experiments, ensure that:
•
The EVOS
™
Onstage Incubator has been set up for use (page 95).
•
The gas inputs have been configured (page 102).
•
The oxygen sensor has been calibrated (page 103).
6.
Adjust brightness and focus for each selected channel as described in “Capture
images” (page 18), then click
Next
.
Note:
When the sample is focused and ready, tighten the stage brakes (page 7)
to prevent the stage from drifting during the time lapse routine.
7.
If you have opted to run
Auto Focus
, select the
Auto Focus Channel
to use.
Note:
In a time lapse routine, you can run the autofocus procedure only in a
single channel (in this example, DAPI). The focal plane identified in this
channel is then used for all other channels.
