EVOS
™
M5000 Imaging System User Guide
31
4.
If needed, click
Target
again to identify further objects
to include in your count (for example, targets that might
appear different).
Note:
For best results, follow these guidelines when identifying target objects:
•
When selecting objects, circle the entire object and include a slight border
around it.
•
To include objects of lower intensity in your count, select dimmer objects
during identification.
•
Circle only one object at a time to help define object size for segmentation.
5.
To distinguish the target from background, click
Backround
, then click and drag to draw a circle (red) in
a background area.
6.
To count closely grouped cells that are touching or overlapping as distinct
objects, select from the
Split Cells
options:
•
None:
Touching or overlapping objects are not counted separately.
•
Shape:
Distinct objects are identified and counted based on shape.
•
Intensity
: Distinct objects are identified and counted based on pixel
intensity.
