EVOS
™
M5000 Imaging System User Guide
19
Adjust brightness
1.
For
Brightness mode
, select
Simple
or
Actual
.
-
Simple
mode
allows you to control
Brightness
as a single
parameter.
-
Actual
mode allows you to adjust
Light
(i.e., LED intensity),
Exposure
, and
Gain
parameters
individually.
2.
Adjust brightness using the
Brightness
controls:
•
In the
Simple
mode, move the
Light
slider to adjust brightness.
Alternatively, enter the desired brightness value (0%
−
100%) directly in the
brightness field.
•
In the
Actual mode
, move the
Light
(LED intensity),
Exposure
, and
Gain
sliders to adjust the brightness parameters individually.
Alternatively, enter the desired value for each parameter (0%
−
100% for
Light, 0
−
4.00 seconds for Exposure, or 1.0
−
8.0 for Gain) directly in the
corresponding field.
Note:
Optimize the brightness parameters as follows:
•
When searching for sample:
Increase
Gain
for a brighter signal and
decrease
Exposure
for faster frame rate during navigation around the
vessel.
•
When capturing image:
Decrease
Gain
to reduce background noise and
increase
Exposure
to regain signal intensity, as needed.
•
For brighter signal:
Increase
Light
intensity for brighter illumination. If
needed, follow by increasing
Gain
.
•
For time lapse imaging:
Increase
Gain
and
Exposure
, and decrease
Light
intensity to reduce photobleaching and phototoxicity.
For example, for overnight time lapse experiments, capture one image
every 30 minutes or less, limit the use of autofocus, and use a channel
other than DAPI for autofocus.
•
In the
Settings
Visuals
tab, select
Highlight Saturated Pixel
to display
the overexposed pixels in the color of your choice. To obtain the
maximum level of brightness without any overexposed areas, dim the
illumination until the highlights disappear.
