3
Tuning
Tuning the Reagent Ion Optics
Thermo Scientific
MS/ETD System Getting Started Guide
19
Manually Tuning the Reagent Ion Source
To manually tune the reagent ion source
1. Open the Tune Plus window; see the instructions on
2. Click the
Display Graph View
(
) button.
3. Turn on the Reagent Ion Source as follows:
a. Choose
Setup > Reagent Ion Source
(
b. Select the
Reagent Ion Source On
check box.
c.
Select the
Filament On
check box.
4. In the Reagent Ion Source dialog box, select the
View Reagent Ion Spectra
check box.
5. In the Tune Plus window, click the
Tune
(
) button to open this dialog box
).
Note
If the reagent vials are not at their target temperature, a message appears:
Reagent Vial NOT At Temperature! Please wait …
The System LED on the ETD module flashes green to indicate that the reagent
vial heaters are turned on but not at their target temperatures (the other heaters
are at their target temperatures).
When the reagent vial heaters reach their target temperatures, the System LED
illuminates solid green.
When the reagent vials reach their target temperature, voltage is applied to the
ETD module ion optics, and when the check box is selected, the filament turns
on and the Filament LED illuminates solid green.