5
Performing an ETD Infusion Experiment
Obtaining an ETD Spectrum for Angiotensin I
36
MS/ETD System Getting Started Guide
Thermo Scientific
4. In the Tune Plus window, click the
Define Scan
(
) button to open this
dialog box (
).
Figure 23.
Define Scan dialog box
5. Under MSn Settings, in the n = 2 row, enter the Parent Mass (
m/z
) of the 3+ charge state
of Angiotensin I.
The molecular weight of Angiotensin I (acetate hydrate) is 1296 Da. The mass-to-charge
ratio of the parent ion [M + 3H]
3+
is 433.0.
6. In the Act. Type list, select
ETD
(
).
Figure 24.
Activation Type list in the Define Scan dialog box
Activation Type = ETD
