5
Performing an ETD Infusion Experiment
Optimizing the Reagent Ion Reaction Time
Thermo Scientific
MS/ETD System Getting Started Guide
39
7. Click the
Reagent Ion Reaction Time
tab (
Figure 27.
Reagent Ion Reaction Time page in the Tune dialog box
8. Click
Start
.
Tune Plus generates a graph showing the intensity of the
m/z
of interest versus reaction
time. The Status area of the window shows a reagent ion reaction time after completing
the tune process.
A message prompts you to accept the optimized value. If you accept the optimized value,
the reagent ion reaction time parameter sets to this optimized value in the Define Scan
dialog box. Otherwise, it returns to its previous value.
The reagent ion reaction time is now optimized based on total ion current (TIC).
To optimize on a particular product ion, in the Tune dialog box under What to Optimize
On (
Product Ion Mass (m/z)
option, enter the
m/z
of the selected
product ion, and then repeat step 7.
