Thermo Scientific ETD, Руководство по началу работы

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5
Performing an ETD Infusion Experiment
Optimizing the Reagent Ion Reaction Time
38 MS/ETD System Getting Started Guide Thermo Scientific
Optimizing the Reagent Ion Reaction Time
The default reagent ion reaction time of 100 ms is appropriate for most analyses. In some
cases obtaining an optimized reagent ion reaction time is helpful for a specific analyte. The
procedures in this section assume that the system generates the reagent ions as described in
“Turning On the Reagent Ion Source” on page 28 .
 To optimize the reagent ion reaction time
1. Turn on ETD activation for the analyte (Angiotensin I, in this case).
2. Open the Tune Plus window; see the instructions on page 13 .
3. Click the Define Scan ( ) button to open this dialog box ( Figure 23 on
page 36 ).
4. Under MSn Settings, in the Parent Mass ( m/z ) box, enter the mass for the analyte.
5. Under MSn Settings, in the Act. Type list, select ETD .
6. In the Tune Plus window, click the Tune ( ) button to open this dialog box.
The Tune dialog box has two additional tabs for tuning ETD ( Figure 26 ).
Figure 26. Tune dialog box for ETD