Thermo Scientific ETD, Руководство по началу работы

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6
Running an Xcalibur Experiment by Using ETD
Data Dependent NL MS
3
Experiment
Thermo Scientific MS/ETD System Getting Started Guide 47
13. In the Tune Plus window, click the Save
(
) button.
14. Browse to the drive :\Xcalibur\methods folder, and specify a file name.
15. Click Save .
Data Dependent NL MS
3
Experiment
 To run a Data Dependent NL MS
3
(ETD) experiment
1. Choose Start > Programs > Xcalibur > Xcalibur ( Figure 28 on page 42 ).
2. Click the Instrument Setup icon ( Figure 29 on page 43 ).
3. In the Available Devices pane, click the LTQ XL MS or LTQ Velos MS icon to open the
New Method page ( Figure 30 on page 44 ).
4. Under Select ETD Experiment Type, click Data Dependent NL MS3 (ETD) to open
this dialog box ( Figure 35 ).
The Initialize Method to Repeat the MS2 Event Using ETD check box is selected as the
default setting.
Figure 35. Data Dependent NL MS3 dialog box
5. In the Analyze Top N Peaks box, enter the number of top peaks.