3
Tuning
Tuning the Reagent Ion Optics
18
MS/ETD System Getting Started Guide
Thermo Scientific
6. In the Tune Plus window, click the
Tune
(
) button to open this dialog box
).
Figure 10.
Automatic page in the Tune dialog box
7. Click
Start.
The system begins automatically tuning the ion optics of the reagent ion source. The
Status area displays the message “Optimization Complete” after completing automatic
tuning. This message also states the percentage change in the reagent ion
signal
(
m/z
= 202) intensity relative to the prior value. A typical reagent signal intensity is about
5E6 to 1E7 in profile mode when the system has been cleaned and the ion volume is new.
8. Repeat Automatic Tune if the percentage change is greater than 20%.
Note
This is an iterative process. At some point there will be no more improvements in
signal intensity.
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