IonPac CS14 Manual
Doc. No. 034848-10
Page 4 of 54
SECTION 6 - TROUBLESHOOTING GUIDE ............................................................................... 42
6.1
High Back Pressure ............................................................................................................................................... 43
6.1.1
Finding the Source of High System Pressure ............................................................................................................ 43
6.1.2
Replacing Column Bed Support Assemblies ............................................................................................................. 44
6.2
Preparation of Eluents ............................................................................................................................................ 45
6.3
A Contaminated Guard or Analytical Column ........................................................................................................ 45
6.4
High Background or Noise ..................................................................................................................................... 45
6.5
A Suppressor Not Suppressing Properly ............................................................................................................... 46
6.6
Poor Peak Resolution ............................................................................................................................................. 47
6.6.1
Loss of Peak Efficiency throughout the Chromatogram ............................................................................................ 47
6.6.2
Loss of Resolution Throughout the Chromatogram Due to Shortened Retention Times ......................................... 47
6.6.3
Loss of Early Eluting Peak Resolution ...................................................................................................................... 48
6.7
Spurious Peaks ....................................................................................................................................................... 48
APPENDIX A - QUALITY ASSURANCE REPORT ....................................................................... 49
APPENDIX B - COLUMN CARE .................................................................................................. 52
Recommended Operating Pressures ................................................................................................................................ 52
Column Start-Up ................................................................................................................................................................ 52
Column Storage ................................................................................................................................................................. 52
Column Conditioning ......................................................................................................................................................... 52
Column Cleanup ................................................................................................................................................................ 52
Column Cleanup Procedure for Polyvalent Cations and Acid-Soluble Contaminants or Transition Metals ........................ 53
Hydrophobic Cations and Organic Contaminants ............................................................................................................... 53
