IonPac CS14 Manual
Doc. No. 034848-10
Page 3 of 54
SECTION 5 - EXAMPLE APPLICATIONS .................................................................................... 16
5.1
Preparation of Eluent Stock Solution Concentrates .............................................................................................. 16
5.1.1
1.0 M Methanesulfonic Acid (MSA) Stock Solution ................................................................................................ 16
5.1.2
1.0 M Trifluoroacetic Acid (TFA) Stock Solution ...................................................................................................... 16
5.1.3
0.1 M 18-Crown-6 Ether Stock Solution .................................................................................................................... 16
5.1.4
Pyrophosphoric Acid ................................................................................................................................................ 17
5.2
Eluent Preparation .................................................................................................................................................. 17
5.3
Production Test Chromatogram ............................................................................................................................. 18
5.4
Isocratic Elution of Ammonium, Group I & II Cations ........................................................................................... 19
5.5
The Effect of Sample Acidity on IonPac CS14 Efficiency ....................................................................................... 20
5.6
Selectivity Control of Magnesium, Manganese and Calcium ................................................................................ 21
5.7
Isocratic Elution of Morpholine and Resolution Enhancement Addition of Solvent to Eluent .............................. 22
5.8
Elution of Morpholine with Mono-, Di- and Trimethylamines With Resolution Enhancement Through Gradient
Elution ...................................................................................................................................................................... 24
5.9
Isocratic Elution of Mono-, Di- and Trimethylamines and Resolution Enhancement through Addition of Solvent to
Eluent ....................................................................................................................................................................... 25
5.10 Elution of Mono-, Di- and Triethylamines With Resolution Enhancement Through the Addition of Solvent to the
Eluent ....................................................................................................................................................................... 26
5.11
Gradient Elution of Cyclohexylamine ..................................................................................................................... 28
5.12 Elution of Aliphatic Monoamines ............................................................................................................................ 29
5.13 Gradient Elution of Alkylamines, Ammonia, Group I & II Cations ....................................................................... 31
5.14 Elution of Di- and Triethanolamines ....................................................................................................................... 33
5.15 The Isocratic Separation of Ethanolamine and the Group I & II Cations .............................................................. 34
5.16 Elution of Substituted Methyl- and Ethylaminoethanols ........................................................................................ 35
5.17 Methanesulfonic Acid Gradient Elution of Alkanolamines with Ammonium, Group I & II Cations .................... 36
5.18 Methanesulfonic acid / 18-Crown-6 Gradient Elution of Alkanolamines with Group I & II Cations .................. 38
5.19 The Effect of Temperature on the Gradient Elution of Diamines with Ammonia, Group I & II Cations ............... 40