IonPac CS14 Manual
Doc. No. 034848-10
Page 40 of 54
5.19 The Effect of Temperature on the Gradient Elution of Diamines with Ammonia, Group I & II
Cations
The determination of many diamines in the presence of ammonia, Group I & II cations can be accomplished by gradient elution
in which methanesulfonic acid varies from 10 mM to 20 mM and the acetonitrile varies from 5% to 15%. The following two
applications demonstrate the effect of temperature on the separation. The efficiency of the peaks is significantly improved by
increasing the column temperature to 60°C centigrade. Note that in gradient analyses, increasing levels of ionic strength will
increase baseline conductivity, while increasing levels of solvent will decrease baseline conductivity.
Sample Loop Volume:
6.25 µL (2-mm), 25 µL (4-mm)
Analytical Column:
IonPac CS14 Analytical Column
Eluent:
See Gradient Program listed below
Eluent Flow Rate:
0.25 mL/min (2-mm), 1.0 mL/min (4-mm)
SRS Suppressor:
Cation Self-Regenerating Suppressor, CSRS ULTRA (2-mm or 4-mm)
External Water Mode
or MMS Suppressor:
Cation MicroMembrane Suppressor, CMMS III (2-mm or 4-mm)
MMS Regenerant:
100 mN TBAOH
(tetrabutylammonium hydroxide)
Expected Background Conductivity:
< 1 µS
Storage Solution:
Eluent
Eluent 1:
Type I DI Water
Eluent Flow Rate:
1.0 mL/min
Eluent 2:
100 mM MSA
(4-mm System)
Eluent 3:
50% Acetonitrile
TIME (min)
%E1
%E2
%E3
Comments
Equilibration
0.0
80
10
10
Equilibrate to 10 mM MSA / 5.0% ACN
7.0
80
10
10
Analysis
0.0
80
10
10
Inject, 10 mM MSA / 5.0% ACN
0.1
80
10
10
Inject Valve to Load Position
10.0
50
20
30
20 mM MSA / 15% ACN
20.0
50
20
30
20 mM MSA / 15% ACN
20.1
80
10
10
10 mM MSA / 5.0% ACN
